MitoTracker Orange CMTMRos

Name: MitoTracker Orange CMTMRos
Brand: MedChemExpress
SKU: HY-D1696
Rating: 4.5 (1 reviews)

Cat. No.: HY-D1696 Purity: 92.08%: FAQs
Data Sheet SDS COA Handling Instructions

Molarity Calculator

MitoTracker Orange CMTMRos is a fluorescent dye that labels mitochondria within live cells utilizing the mitochondrial membrane potential (Ex/Em: 551/576 nm).

For research use only. We do not sell to patients.

MitoTracker Orange CMTMRos Chemical Structure

CAS No. : 199116-50-2

Size	Price	Stock	Quantity
1 mg	USD 280	In-stock	Estimated Time of Arrival: December 31
5 mg	USD 980	In-stock	Estimated Time of Arrival: December 31
10 mg	USD 1650	In-stock	Estimated Time of Arrival: December 31
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100 mg		Get quote

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Biological Activity
Purity & Documentation
References
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Description

MitoTracker Orange CMTMRos is a fluorescent dye that labels mitochondria within live cells utilizing the mitochondrial membrane potential (Ex/Em: 551/576 nm)^[1].

IC₅₀ & Target

Mitochondria^[1]

In Vitro

Guidelines^[1] (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs).
Labeling of Cells:
For live cells, follow Steps 1–7. For fixed cells, follow Steps 8–13.
1. Prepare a labeling solution at 25–500 nM in the desired cell culture medium, and warm it to the cell culture temperature.
2. Aspirate the culture medium from cells grown on coverslips.
3. Immerse the cells in the labeling medium for 15–45 min at 37?C.
4. Aspirate the labeling medium, and rinse the cells three times with culture medium.
5. Mount the cells.
6. Check the cells using a fluorescence microscope. If the fluorescence staining is too low, try one of the following options:
i. Incubate for an additional 30 min in the normal culture medium to allow the thiol conjugation to proceed.
ii. Try a higher initial concentration of the labeling solution.
7. When the fluorescence is sufficient, image the cells.
8. Carry out Steps 1–4, but use a labeling solution at a working concentration between 100 and 1000 nM.
9. Rinse the cells three times with PBS+.
10. Fix the cells for 10 min in 3.7% formaldehyde in PBS (pH 7.4).
11. Aspirate and rinse the cells three times for 5 min each in PBS.
12. Mount the coverslips.
13. Image the cells.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

427.37

Formula

C₂₄H₂₄Cl₂N₂O

CAS No.

199116-50-2

Appearance

Solid

Color

Brown to black

Emission (Em)

576

Excitation (Ex)

554

SMILES

CN(C)C(C=C1)=CC2=C1C(C3=CC=C(CCl)C=C3)=C(C=C/4)C(O2)=CC4=[N+](C)/C.[Cl-]

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, sealed storage, away from moisture and light

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

Solvent & Solubility

In Vitro:

DMSO : 31.25 mg/mL (73.12 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.3399 mL	11.6995 mL	23.3989 mL
5 mM	0.4680 mL	2.3399 mL	4.6798 mL
10 mM	0.2340 mL	1.1699 mL	2.3399 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

Purity & Documentation

Purity: 92.08%

Select Batch:

Data Sheet (274 KB) SDS (252 KB)

COA (250 KB) HNMR (266 KB) RP-HPLC (251 KB)

Handling Instructions (2659 KB)

References

[1]. Chazotte B. Labeling mitochondria with MitoTracker dyes. Cold Spring Harb Protoc. 2011 Aug 1;2011(8):990-2. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	2.3399 mL	11.6995 mL	23.3989 mL	58.4973 mL
	5 mM	0.4680 mL	2.3399 mL	4.6798 mL	11.6995 mL
	10 mM	0.2340 mL	1.1699 mL	2.3399 mL	5.8497 mL
	15 mM	0.1560 mL	0.7800 mL	1.5599 mL	3.8998 mL
	20 mM	0.1170 mL	0.5850 mL	1.1699 mL	2.9249 mL
	25 mM	0.0936 mL	0.4680 mL	0.9360 mL	2.3399 mL
	30 mM	0.0780 mL	0.3900 mL	0.7800 mL	1.9499 mL
	40 mM	0.0585 mL	0.2925 mL	0.5850 mL	1.4624 mL
	50 mM	0.0468 mL	0.2340 mL	0.4680 mL	1.1699 mL
	60 mM	0.0390 mL	0.1950 mL	0.3900 mL	0.9750 mL