MKA031

Cat. No.: HY-149308 Purity: 98.69%: FAQs
Data Sheet SDS COA Handling Instructions Technical Support

MKA031 (compound 6y) is a non-competitive MIF inhibitor with an IC₅₀ value of 1.7 μM. MKA031 (compound 6y) interferes with MIF/AIF interaction, MIF nuclear translocation, and N-methyl-N'-nitro-N-nitrosoguanidine (MNNG)-induced dependent cell death. MKA031 can be used in the study of chronic hepatitis C virus infection.

For research use only. We do not sell to patients.

MKA031 Chemical Structure

CAS No. : 2951012-00-1

Size	Stock	Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	In-stock	Estimated Time of Arrival: December 31
Solid
5 mg	In-stock	Estimated Time of Arrival: December 31
10 mg	In-stock	Estimated Time of Arrival: December 31
25 mg	In-stock	Estimated Time of Arrival: December 31
50 mg	In-stock	Estimated Time of Arrival: December 31
100 mg	In-stock	Estimated Time of Arrival: December 31
200 mg	Get quote
500 mg	Get quote

or Bulk Inquiry

* Please select Quantity before adding items.

This product is a controlled substance and not for sale in your territory.

Featured Recommendations

•Related Small Molecules:

•Related Proteins:

Biological Activity
Purity & Documentation
References
Customer Review

Description

In Vitro

MKA031 (compound 6y) (1-100 μl; 3 h) prevents MNNG-Induced Cell Death^[1]. MKA031 (compound 6y) (100 μl;3 h) protects Genomic DNA by Blocking the Recruitment of MIF to AIF^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay^[1]

Cell Line:	Hela cells
Concentration:	1-100 μM
Incubation Time:	3 h
Result:	Protected HeLa cells from MNNG-induced parthanatos in a dose dependent manner with an EC₅₀ of 7.7 μM.

Immunofluorescence^[1]

Cell Line:	Hela cells
Concentration:	100 μM
Incubation Time:	3 h
Result:	Interfered with the MIF/AIF interaction and blocks MIF nuclear translocation.

Molecular Weight

403.46

Formula

C₂₁H₁₇N₅O₂S

CAS No.

2951012-00-1

Appearance

Solid

Color

Off-white to light yellow

SMILES

O=C(NC1=CC=CC=C1)C2=CC=C(C=C2)N3N=NC(C(NCC4=CC=CS4)=O)=C3

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
In solvent	-80°C	6 months
	-20°C	1 month

Solvent & Solubility

In Vitro:

DMSO : 25 mg/mL (61.96 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.4786 mL	12.3928 mL	24.7856 mL
5 mM	0.4957 mL	2.4786 mL	4.9571 mL
10 mM	0.2479 mL	1.2393 mL	2.4786 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

Purity & Documentation

Purity: 98.69%

Select Batch:

Data Sheet (271 KB) SDS (251 KB)

COA (246 KB) HNMR (254 KB) LCMS (111 KB)

Handling Instructions (2659 KB)

References

[1]. Chen D, et al. Allosteric Inhibitors of Macrophage Migration Inhibitory Factor (MIF) Interfere with Apoptosis-Inducing Factor (AIF) Co-Localization to Prevent Parthanatos. J Med Chem. 2023 Jul 13;66(13):8767-8781. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	2.4786 mL	12.3928 mL	24.7856 mL	61.9640 mL
	5 mM	0.4957 mL	2.4786 mL	4.9571 mL	12.3928 mL
	10 mM	0.2479 mL	1.2393 mL	2.4786 mL	6.1964 mL
	15 mM	0.1652 mL	0.8262 mL	1.6524 mL	4.1309 mL
	20 mM	0.1239 mL	0.6196 mL	1.2393 mL	3.0982 mL
	25 mM	0.0991 mL	0.4957 mL	0.9914 mL	2.4786 mL
	30 mM	0.0826 mL	0.4131 mL	0.8262 mL	2.0655 mL
	40 mM	0.0620 mL	0.3098 mL	0.6196 mL	1.5491 mL
	50 mM	0.0496 mL	0.2479 mL	0.4957 mL	1.2393 mL
	60 mM	0.0413 mL	0.2065 mL	0.4131 mL	1.0327 mL

MKA031 Related Classifications

Infection

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

MKA0312951012-00-1MKA 031MKA-031Macrophage migration inhibitory factor (MIF)Hepatitis CMNNGMIF/AIFparthanatosInhibitorinhibitorinhibit

Your Recently Viewed Products:

Product Name

Requested quantity *

Applicant Name *

Salutation

Email Address *

Phone Number *

Department

Organization Name *

City

State

Country or Region *

Remarks

Product Name

Lot #

Applicant Name *

Email Address *

Phone Number

Department *

Organization Name *

Country or Region *

Remarks

Name
Email *

	Sorry, but the email address you supplied was invalid.

MKA031

MKA031 Related Antibodies

Featured Recommendations

•Related Small Molecules:

•Related Proteins:

Biological Activity

Purity & Documentation

References

Customer Review

MKA031 Related Antibodies

Molarity Calculator

Dilution Calculator