2. Anti-infection
  3. Bacterial
  4. ML328

ML328 is a selective inhibitor of bacterial AddAB and RecBCD helicase-nucleases with IC50 values of 26 and 5.1 μM, respectively. ML328 is a gyrase inhibitor. ML328 strongly inhibits the growth of E. coli in the presence of phage. ML328 can be used for the research of bacterial infection.

For research use only. We do not sell to patients.

ML328 Chemical Structure

ML328 Chemical Structure

CAS No. : 634175-34-1

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5 mg USD 180 In-stock
10 mg USD 290 In-stock
25 mg USD 550 In-stock
50 mg USD 880 In-stock
100 mg USD 1400 In-stock
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Based on 1 publication(s) in Google Scholar

ML328 Related Antibodies

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Description

ML328 is a selective inhibitor of bacterial AddAB and RecBCD helicase-nucleases with IC50 values of 26 and 5.1 μM, respectively. ML328 is a gyrase inhibitor. ML328 strongly inhibits the growth of E. coli in the presence of phage. ML328 can be used for the research of bacterial infection[1][2].

Cellular Effect
Cell Line Type Value Description References
MDA-MB-231 IC50
0.9 μM
Compound: 11
Inhibition of human autotaxin isolated from MDA-MB-231 cells assessed as blockade of FS3 substrate hydrolysis by FRET assay
Inhibition of human autotaxin isolated from MDA-MB-231 cells assessed as blockade of FS3 substrate hydrolysis by FRET assay
[PMID: 20041668]
Sf9 IC50
900 μM
Compound: Hoeglund 11
Inhibition of C-terminal FLAG tagged human recombinant ATX expressed in insect sf9 cells using FS-3 as substrate measured every 2 mins for 60 mins by FRET assay
Inhibition of C-terminal FLAG tagged human recombinant ATX expressed in insect sf9 cells using FS-3 as substrate measured every 2 mins for 60 mins by FRET assay
[PMID: 27544588]
In Vitro

ML328 (0.1-1000 μM) shows AddAB and RecBCD nuclease inhibitory activities with IC50 values of 26 and 5.1 μM, respectively[1].
ML328 pathway-specifically inhibits of (high-frequency recombination) Hfr for RecBCD, RecF and RecE pathways[1].
ML328 shows RecBCD inhibitory effects with IC50 values of 3 μΜ for nuclease and Chi-cutting with purified enzyme, 0.3 μΜ for Hfr recombination and 5 μΜ for phage λ recombination promoted by RecBCD[2].
ML328 inhibits E. coli RecBCD, H. pylori AddAB, M. smeg AddAB and M. smeg RecBCD with IC50 values of 4.6, 16, 2.4 and 5.5 μM, respectively[2].
ML328 (25 μΜ; 2 h) reduces the frequency of H2O2-induced mutation in E. coli[2].
ML328 (25 μΜ; 1 h) reduces the requency of H2O2-induced mutation to valine-resistance (valineR) in E. coli[2].
ML328 (50 μΜ) slightly inhibits E. coli growth but strongly inhibits E. coli at the presence of phage[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

ML328 Related Antibodies
Molecular Weight

506.50

Formula

C22H21F3N6O3S
CAS No.
Appearance

Solid

Color

White to off-white

SMILES

O=C(C1=CN(CC)C2=NC(N3CCN(C(NC4=CC=CC(C(F)(F)F)=C4)=S)CC3)=NC=C2C1=O)O
Shipping

Room temperature in continental US; may vary elsewhere.
Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
Solvent & Solubility
In Vitro: 

DMSO : 25 mg/mL (49.36 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 1.9743 mL 9.8717 mL 19.7433 mL
5 mM 0.3949 mL 1.9743 mL 3.9487 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 1.9743 mL 9.8717 mL 19.7433 mL 49.3583 mL
5 mM 0.3949 mL 1.9743 mL 3.9487 mL 9.8717 mL
10 mM 0.1974 mL 0.9872 mL 1.9743 mL 4.9358 mL
15 mM 0.1316 mL 0.6581 mL 1.3162 mL 3.2906 mL
20 mM 0.0987 mL 0.4936 mL 0.9872 mL 2.4679 mL
25 mM 0.0790 mL 0.3949 mL 0.7897 mL 1.9743 mL
30 mM 0.0658 mL 0.3291 mL 0.6581 mL 1.6453 mL
40 mM 0.0494 mL 0.2468 mL 0.4936 mL 1.2340 mL
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ML328 Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

ML328634175-34-1ML 328ML-328BacterialbacterialHfrChi-cuttingE. coliphageInhibitorinhibitorinhibit

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