ML336

Cat. No.: HY-12928 Purity: 99.09%: FAQs
Data Sheet SDS COA Handling Instructions

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ML336 is quinazolinone-based inhibitor against venezuelan equine encephalitis virus (VEEV), with IC₅₀s of 32, 20, and 42 nM for VEEV TC-83 CPE , VEEV V3526 CPE, VEEV Wild Type CPE, respectively. ML336 potently inhibits a VEEV-induced cytopathic effect in three strains of the virus (TC-83, V3526, and wild type Trinidad donkey) in the low nanomolar range.

For research use only. We do not sell to patients.

ML336 Chemical Structure

CAS No. : 1613465-33-0

Size	Price	Stock	Quantity
1 mg	USD 150	In-stock	Estimated Time of Arrival: December 31
5 mg	USD 300	In-stock	Estimated Time of Arrival: December 31
10 mg	USD 450	In-stock	Estimated Time of Arrival: December 31
25 mg	USD 770	In-stock	Estimated Time of Arrival: December 31
50 mg		Get quote
100 mg		Get quote

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Based on 1 publication(s) in Google Scholar

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References
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Description

Cellular Effect

Cell Line	Type	Value	Description	References
Vero 76	EC₅₀	> 50 μM Compound: 45, ML336	Antiviral activity against Venezuelan equine encephalitis virus TC83 containing non-structural protein 4 Q210K mutant infected in Vero 76 cells assessed as reduction in virus-induced cytopathic effect after 2 days by Celltiter-Glo reagent based assay Antiviral activity against Venezuelan equine encephalitis virus TC83 containing non-structural protein 4 Q210K mutant infected in Vero 76 cells assessed as reduction in virus-induced cytopathic effect after 2 days by Celltiter-Glo reagent based assay	[PMID: 25244572]
Vero 76	CC₅₀	> 50 μM Compound: 45, ML336	Cytotoxicity against african green monkey Vero 76 cells after 3 days by Celltiter-Glo reagent based assay Cytotoxicity against african green monkey Vero 76 cells after 3 days by Celltiter-Glo reagent based assay	[PMID: 25244572]
Vero 76	EC₅₀	0.02 μM Compound: 45, ML336	Antiviral activity against Venezuelan equine encephalitis virus V3526 infected in Vero 76 cells assessed as reduction in virus-induced cytopathic effect after 2 days by Celltiter-Glo reagent based assay Antiviral activity against Venezuelan equine encephalitis virus V3526 infected in Vero 76 cells assessed as reduction in virus-induced cytopathic effect after 2 days by Celltiter-Glo reagent based assay	[PMID: 25244572]
Vero 76	EC₅₀	0.03 μM Compound: 45, ML336	Antiviral activity against Venezuelan equine encephalitis virus TC83 infected in Vero 76 cells assessed as reduction in virus-induced cytopathic effect after 2 days by Celltiter-Glo reagent based assay Antiviral activity against Venezuelan equine encephalitis virus TC83 infected in Vero 76 cells assessed as reduction in virus-induced cytopathic effect after 2 days by Celltiter-Glo reagent based assay	[PMID: 25244572]
Vero 76	EC₅₀	0.04 μM Compound: 45, ML336	Antiviral activity against Venezuelan equine encephalitis virus TrD infected in Vero 76 cells assessed as reduction in virus-induced cytopathic effect after 2 days by Celltiter-Glo reagent based assay Antiviral activity against Venezuelan equine encephalitis virus TrD infected in Vero 76 cells assessed as reduction in virus-induced cytopathic effect after 2 days by Celltiter-Glo reagent based assay	[PMID: 25244572]
Vero 76	EC₅₀	0.17 μM Compound: 45, ML336	Antiviral activity against Venezuelan equine encephalitis virus TC83 infected in Vero 76 cells assessed as reduction in viral titer by crystal violet staining based microplaque assay relative to untreated control Antiviral activity against Venezuelan equine encephalitis virus TC83 infected in Vero 76 cells assessed as reduction in viral titer by crystal violet staining based microplaque assay relative to untreated control	[PMID: 25244572]
Vero 76	EC₅₀	17 μM Compound: 45, ML336	Antiviral activity against Venezuelan equine encephalitis virus TC83 containing non-structural protein 2 Y102C mutant infected in Vero 76 cells assessed as reduction in virus-induced cytopathic effect after 2 days by Celltiter-Glo reagent based assay Antiviral activity against Venezuelan equine encephalitis virus TC83 containing non-structural protein 2 Y102C mutant infected in Vero 76 cells assessed as reduction in virus-induced cytopathic effect after 2 days by Celltiter-Glo reagent based assay	[PMID: 25244572]
Vero 76	EC₅₀	19 μM Compound: 45, ML336	Antiviral activity against Venezuelan equine encephalitis virus TC83 containing non-structural protein 2 D116N mutant infected in Vero 76 cells assessed as reduction in virus-induced cytopathic effect after 2 days by Celltiter-Glo reagent based assay Antiviral activity against Venezuelan equine encephalitis virus TC83 containing non-structural protein 2 D116N mutant infected in Vero 76 cells assessed as reduction in virus-induced cytopathic effect after 2 days by Celltiter-Glo reagent based assay	[PMID: 25244572]
Vero 76	EC₅₀	19 μM Compound: 45, ML336	Antiviral activity against Venezuelan equine encephalitis virus V3526 containing non-structural protein 2 Y102C mutant infected in Vero 76 cells assessed as reduction in virus-induced cytopathic effect after 2 days by Celltiter-Glo reagent based assay Antiviral activity against Venezuelan equine encephalitis virus V3526 containing non-structural protein 2 Y102C mutant infected in Vero 76 cells assessed as reduction in virus-induced cytopathic effect after 2 days by Celltiter-Glo reagent based assay	[PMID: 25244572]
Vero 76	EC₅₀	66 μM Compound: 45, ML336	Antiviral activity against Venezuelan equine encephalitis virus V3526 containing non-structural protein 4 Q210K mutant infected in Vero 76 cells assessed as reduction in virus-induced cytopathic effect after 2 days by Celltiter-Glo reagent based assay Antiviral activity against Venezuelan equine encephalitis virus V3526 containing non-structural protein 4 Q210K mutant infected in Vero 76 cells assessed as reduction in virus-induced cytopathic effect after 2 days by Celltiter-Glo reagent based assay	[PMID: 25244572]

Molecular Weight

367.40

Formula

C₁₉H₂₁N₅O₃

CAS No.

1613465-33-0

Appearance

Solid

Color

Off-white to yellow

SMILES

O=C(NC1=CC=CC=C1)C2=CC([N+]([O-])=O)=CC=C2/N=C3N(C)CCN(C)C/3

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

Solvent & Solubility

In Vitro:

DMSO : 33.33 mg/mL (90.72 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.7218 mL	13.6091 mL	27.2183 mL
5 mM	0.5444 mL	2.7218 mL	5.4437 mL
10 mM	0.2722 mL	1.3609 mL	2.7218 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

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(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

Purity & Documentation

Purity: 99.09%

Select Batch:

Data Sheet (274 KB) SDS (252 KB)

COA (252 KB) HNMR (408 KB) RP-HPLC (265 KB) LCMS (96 KB)

Handling Instructions (2659 KB)

References

[1]. Chung D, et al. ML336: Development of Quinazolinone-Based Inhibitors Against Venezuelan Equine Encephalitis Virus (VEEV). 2012 Dec 17 [updated 2013 Nov 7]. In: Probe Reports from the NIH Molecular Libraries Program [Internet]. Bethesda (MD): National Cent [Content Brief]

[2]. Donghoon Chung 1, et al. ML336: Development of Quinazolinone-Based Inhibitors Against Venezuelan Equine Encephalitis Virus (VEEV). [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	2.7218 mL	13.6091 mL	27.2183 mL	68.0457 mL
	5 mM	0.5444 mL	2.7218 mL	5.4437 mL	13.6091 mL
	10 mM	0.2722 mL	1.3609 mL	2.7218 mL	6.8046 mL
	15 mM	0.1815 mL	0.9073 mL	1.8146 mL	4.5364 mL
	20 mM	0.1361 mL	0.6805 mL	1.3609 mL	3.4023 mL
	25 mM	0.1089 mL	0.5444 mL	1.0887 mL	2.7218 mL
	30 mM	0.0907 mL	0.4536 mL	0.9073 mL	2.2682 mL
	40 mM	0.0680 mL	0.3402 mL	0.6805 mL	1.7011 mL
	50 mM	0.0544 mL	0.2722 mL	0.5444 mL	1.3609 mL
	60 mM	0.0454 mL	0.2268 mL	0.4536 mL	1.1341 mL
	80 mM	0.0340 mL	0.1701 mL	0.3402 mL	0.8506 mL

ML336 Related Classifications

Infection

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

ML3361613465-33-0ML 336ML-336Virus Proteasevenezuelan equine encephalitis virusVEEVVEEV TC-83 CPE VEEV V3526 CPEVEEV Wild Type CPEInhibitorinhibitorinhibit

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ML336

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Complete Stock Solution Preparation Table

ML336 Related Classifications

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