MPT0B002

Cat. No.: HY-122151: FAQs
Data Sheet Handling Instructions

Molarity Calculator

MPT0B002 is a potent microtubule inhibitor with anticancer activities. MPT0B002 disrupts tubulin polymerization, induces apoptosis, and arrests cell cycle at the G2/M phase.

For research use only. We do not sell to patients.

MPT0B002 Chemical Structure

CAS No. : 946077-08-3

Size		Stock
MOQ		Minimum order quantity
50 mg		Get quote
100 mg		Get quote
250 mg		Get quote
Other size		Get quote

Synthetic products have potential research and development risk.

* Please select Quantity before adding items.

This product is a controlled substance and not for sale in your territory.

Featured Recommendations

•Related Small Molecules:

•Related Proteins:

Biological Activity
Purity & Documentation
References
Customer Review

Description

MPT0B002 is a potent microtubule inhibitor with anticancer activities. MPT0B002 disrupts tubulin polymerization, induces apoptosis, and arrests cell cycle at the G2/M phase^[1].

Cellular Effect

Cell Line	Type	Value	Description	References
A549	IC₅₀	0.12 μM Compound: XIII	Cytotoxicity against human A549 cells after 72 hrs by XTT assay Cytotoxicity against human A549 cells after 72 hrs by XTT assay	[PMID: 19939521]
A549	IC₅₀	0.12 μM Compound: 4d	Cytotoxicity against human A549 cells after 72 hrs by XTT assay Cytotoxicity against human A549 cells after 72 hrs by XTT assay	[PMID: 19647439]
A549	IC₅₀	0.12 μM Compound: 7	Cytotoxicity against human A549 cells after 72 hrs by XTT method Cytotoxicity against human A549 cells after 72 hrs by XTT method	[PMID: 18502133]
A549	IC₅₀	190 nM Compound: 4	Cytotoxicity against human A549 cells assessed as growth inhibition after 72 hrs by XTT assay Cytotoxicity against human A549 cells assessed as growth inhibition after 72 hrs by XTT assay	[PMID: 23470139]
HeLa	IC₅₀	0.034 μM Compound: XIII	Cytotoxicity against human HeLa cells after 72 hrs by XTT assay Cytotoxicity against human HeLa cells after 72 hrs by XTT assay	[PMID: 19939521]
HeLa	IC₅₀	0.034 μM Compound: 7	Cytotoxicity against human HeLa cells after 72 hrs by XTT method Cytotoxicity against human HeLa cells after 72 hrs by XTT method	[PMID: 18502133]
HeLa	IC₅₀	0.035 μM Compound: 4d	Cytotoxicity against human HeLa cells after 72 hrs by XTT assay Cytotoxicity against human HeLa cells after 72 hrs by XTT assay	[PMID: 19647439]
HeLa	IC₅₀	25 nM Compound: 4	Cytotoxicity against human HeLa cells assessed as growth inhibition after 72 hrs by XTT assay Cytotoxicity against human HeLa cells assessed as growth inhibition after 72 hrs by XTT assay	[PMID: 23470139]
HL-60	IC₅₀	27 nM Compound: 4	Cytotoxicity against human HL60 cells assessed as growth inhibition after 72 hrs by XTT assay Cytotoxicity against human HL60 cells assessed as growth inhibition after 72 hrs by XTT assay	[PMID: 23470139]
HT-29	IC₅₀	10.5 nM Compound: 14	Growth inhibition of human HT29 cells after 72 hrs by methylene blue dye assay Growth inhibition of human HT29 cells after 72 hrs by methylene blue dye assay	[PMID: 17685504]
HT-29	IC₅₀	27 nM Compound: 4	Cytotoxicity against human HT-29 cells assessed as growth inhibition after 72 hrs by XTT assay Cytotoxicity against human HT-29 cells assessed as growth inhibition after 72 hrs by XTT assay	[PMID: 23470139]
KB	IC₅₀	13.6 nM Compound: 14	Growth inhibition of human KB cells after 72 hrs by methylene blue dye assay Growth inhibition of human KB cells after 72 hrs by methylene blue dye assay	[PMID: 17685504]
MKN-45	IC₅₀	11.2 nM Compound: 14	Growth inhibition of human MKN45 cells after 72 hrs by methylene blue dye assay Growth inhibition of human MKN45 cells after 72 hrs by methylene blue dye assay	[PMID: 17685504]
NCI-H460	IC₅₀	14.1 nM Compound: 14	Growth inhibition of human H460 cells after 72 hrs by methylene blue dye assay Growth inhibition of human H460 cells after 72 hrs by methylene blue dye assay	[PMID: 17685504]
TSGH	IC₅₀	13.4 nM Compound: 14	Growth inhibition of human TSGH cells after 72 hrs by methylene blue dye assay Growth inhibition of human TSGH cells after 72 hrs by methylene blue dye assay	[PMID: 17685504]

In Vitro

MPT0B002 (1-1000 nM; 48 h) induces G2/M-phase cell cycle arrest^[1].
MPT0B002 (100 nM; 24 h) inhibits cell viability in a dose-dependent manner in K562, BaF3/p210, and BaF3/T315I cells^[1].
MPT0B002 (100 nM; 48 h) induces apoptosis and can downregulate both Bcr-Abl and Bcr-Abl-T315I mRNA expressions and protein levels and the downstream signaling pathways^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay^[1]

Cell Line:	K562, BaF3/p210, and BaF3/T315I cells
Concentration:	1 nM, 10 nM, 100 nM, 1000 nM
Incubation Time:	48 h
Result:	Inhibited cell viability in a dose-dependent manner.

Cell Cycle Analysis^[1]

Cell Line:	K562, BaF3/p210, and BaF3/T315I cells
Concentration:	100 nM
Incubation Time:	24 h
Result:	Induced G2/M-phase cell cycle arrest.

Apoptosis Analysis^[1]

Cell Line:	K562, BaF3/p210, and BaF3/T315I cells
Concentration:	100 nM
Incubation Time:	48 h
Result:	Induced apoptosis and caspase-3 pathway.

Western Blot Analysis^[1]

Cell Line:	K562, BaF3/p210, and BaF3/T315I cells
Concentration:	100 nM
Incubation Time:	48 h
Result:	Significantly reduced the expression level of Bcr-Abl, and reduced the phosphorylations of Crkl and Stat5.

Molecular Weight

325.36

Formula

C₁₉H₁₉NO₄

CAS No.

946077-08-3

SMILES

O=C(C1=CC(OC)=C(C(OC)=C1)OC)C2=CC3=C(C=C2)N(C=C3)C

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Please store the product under the recommended conditions in the Certificate of Analysis.

Purity & Documentation

Handling Instructions (2659 KB)

References

[1]. Yi-Yen Yeh, et al. MPT0B002, a novel microtubule inhibitor, downregulates T315I mutant Bcr-Abl and induces apoptosis of imatinib-resistant chronic myeloid leukemia cells. Invest New Drugs. 2017 Aug;35(4):427-435. [Content Brief]

MPT0B002 Related Classifications

Molarity Calculator
Dilution Calculator

The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass		Concentration		Volume		Molecular Weight *
	=		×		×

The dilution calculator equation

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)	×	Volume _(start)	=	Concentration _(final)	×	Volume _(final)
	×		=		×
C1		V1		C2		V2

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

MPT0B002946077-08-3Microtubule/TubulinApoptosismicrotubule inhibitoranticancer activitiestubulin polymerizationinduces apoptosisInhibitorinhibitorinhibit

Your Recently Viewed Products:

Product Name

Salutation

Applicant Name *

Email Address *

Phone Number *

Organization Name *

Department *

Requested quantity *

Country or Region *

Remarks