N-Phenylnaphthalen-1-amine

Cat. No.: HY-W009756: FAQs
Data Sheet Handling Instructions Technical Support

N-Phenylnaphthalen-1-amine is a fluorescence probe for odorant-binding proteins (OBP) with a dissociation constant of 1.67 μM. N-Phenylnaphthalen-1-amine exhibits an excitation wavelength of 337 nM and an emission wavelength of 407 nM.

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N-Phenylnaphthalen-1-amine Chemical Structure

CAS No. : 90-30-2

Size	Stock	Quantity

Free Sample (0.1 - 0.5 mg)	Apply Now
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	In-stock	Estimated Time of Arrival: December 31
Solid
25 g	In-stock	Estimated Time of Arrival: December 31
50 g	Get quote

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Description

In Vitro

Odorant Binding Protein (OBP) Binding Affinity Assay^[1]
1. Materials Preparation
Fluorescent probe: N-phenyl-1-naphthylamine, dissolved in methanol (MeOH) to prepare a 1 mM stock solution
Protein solution: 2 μM odorant binding protein (OBP), dissolved in 50 mM Tris-HCl buffer (pH 7.4)
Experimental temperature: 25 °C
Fluorescence spectrophotometer: equipped with a 1 cm quartz cuvette, excitation and emission slit widths set to 5 nm
Detection wavelengths:
Excitation wavelength (λ_ex): 337 nm
Emission wavelength (λ_em): 407 nm
2. Fluorescence Binding Assay Procedure
Place 2 μM odorant binding protein solution into the cuvette.
Gradually add the N-phenyl-1-naphthylamine stock solution (1 mM in methanol), mixing gently after each addition.
Set the final concentration range to 1-20 μM.
After each addition, immediately record the fluorescence emission spectrum (range 380-460 nm).
Measure and record the emission intensity at 407 nm for plotting the binding curve.
3. Data Analysis
Plot fluorescence intensity against the concentration of free N-phenyl-1-naphthylamine.
Use a Scatchard plot for linearization and calculation of the binding constant.
Assume a 1:1 binding ratio and 100% protein activity.
The dissociation constant (K_d) of N-phenyl-1-naphthylamine with loc-OBP1 is 1.67 μM.
4. Competitive Binding Assay
Use a fixed concentration of 2 μM odorant binding protein and 1 μM N-phenyl-1-naphthylamine.
Add test ligands (dissolved in methanol) at final concentrations typically ranging from 1-20 μM.
Monitor the decrease in fluorescence intensity and plot the competitive binding curve.
Calculate the IC₅₀, and then convert it to the dissociation constant (K_d) of the test ligand.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

219.29

Formula

C₁₆H₁₃N

CAS No.

90-30-2

Appearance

Solid

Color

Light brown to brown

SMILES

C12=CC=CC=C1C(NC3=CC=CC=C3)=CC=C2

Initial Source

Locusta migratoria

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvent & Solubility

In Vitro:

DMSO : ≥ 100 mg/mL (456.02 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	4.5602 mL	22.8012 mL	45.6023 mL
5 mM	0.9120 mL	4.5602 mL	9.1205 mL
10 mM	0.4560 mL	2.2801 mL	4.5602 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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This equation is commonly abbreviated as: C₁V₁ = C₂V₂

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Purity & Documentation

Handling Instructions (2659 KB)

References

[1]. Ban L, et al., Biochemical characterization and bacterial expression of an odorant-binding protein from Locusta migratoria. Cell Mol Life Sci. 2003 Feb;60(2):390-400. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	4.5602 mL	22.8012 mL	45.6023 mL	114.0058 mL
	5 mM	0.9120 mL	4.5602 mL	9.1205 mL	22.8012 mL
	10 mM	0.4560 mL	2.2801 mL	4.5602 mL	11.4006 mL
	15 mM	0.3040 mL	1.5201 mL	3.0402 mL	7.6004 mL
	20 mM	0.2280 mL	1.1401 mL	2.2801 mL	5.7003 mL
	25 mM	0.1824 mL	0.9120 mL	1.8241 mL	4.5602 mL
	30 mM	0.1520 mL	0.7600 mL	1.5201 mL	3.8002 mL
	40 mM	0.1140 mL	0.5700 mL	1.1401 mL	2.8501 mL
	50 mM	0.0912 mL	0.4560 mL	0.9120 mL	2.2801 mL
	60 mM	0.0760 mL	0.3800 mL	0.7600 mL	1.9001 mL
	80 mM	0.0570 mL	0.2850 mL	0.5700 mL	1.4251 mL
	100 mM	0.0456 mL	0.2280 mL	0.4560 mL	1.1401 mL