2. Antibody-drug Conjugate/ADC Related
  3. ADC Linker
  4. N3-C2-NHS ester

N3-C2-NHS ester is a noncleavable ADC linker used in the synthesis of antibody-drug conjugates (ADCs). N3-C2-NHS ester is a click chemistry reagent, it contains an Azide group and can undergo copper-catalyzed azide-alkyne cycloaddition reaction (CuAAc) with molecules containing Alkyne groups. It can also undergo strain-promoted alkyne-azide cycloaddition (SPAAC) reactions with molecules containing DBCO or BCN groups.

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N3-C2-NHS ester Chemical Structure

N3-C2-NHS ester Chemical Structure

CAS No. : 850180-76-6

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25 mg USD 110 In-stock
50 mg USD 175 In-stock
100 mg USD 280 In-stock
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Based on 1 publication(s) in Google Scholar

N3-C2-NHS ester Related Antibodies

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Description

N3-C2-NHS ester is a noncleavable ADC linker used in the synthesis of antibody-drug conjugates (ADCs). N3-C2-NHS ester is a click chemistry reagent, it contains an Azide group and can undergo copper-catalyzed azide-alkyne cycloaddition reaction (CuAAc) with molecules containing Alkyne groups. It can also undergo strain-promoted alkyne-azide cycloaddition (SPAAC) reactions with molecules containing DBCO or BCN groups.

IC50 & Target

Non-cleavable Linker

 

Molecular Weight

212.16

Formula

C7H8N4O4
CAS No.
Appearance

Oil

Color

Colorless to off-white

SMILES

O=C(ON1C(CCC1=O)=O)CCN=[N+]=[N-]
Shipping

Room temperature in continental US; may vary elsewhere.
Storage
Pure form -20°C 3 years
In solvent -80°C 6 months
-20°C 1 month
Solvent & Solubility
In Vitro: 

DMSO : 100 mg/mL (471.34 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 4.7134 mL 23.5671 mL 47.1342 mL
5 mM 0.9427 mL 4.7134 mL 9.4268 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
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Volume
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Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

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Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 4.7134 mL 23.5671 mL 47.1342 mL 117.8356 mL
5 mM 0.9427 mL 4.7134 mL 9.4268 mL 23.5671 mL
10 mM 0.4713 mL 2.3567 mL 4.7134 mL 11.7836 mL
15 mM 0.3142 mL 1.5711 mL 3.1423 mL 7.8557 mL
20 mM 0.2357 mL 1.1784 mL 2.3567 mL 5.8918 mL
25 mM 0.1885 mL 0.9427 mL 1.8854 mL 4.7134 mL
30 mM 0.1571 mL 0.7856 mL 1.5711 mL 3.9279 mL
40 mM 0.1178 mL 0.5892 mL 1.1784 mL 2.9459 mL
50 mM 0.0943 mL 0.4713 mL 0.9427 mL 2.3567 mL
60 mM 0.0786 mL 0.3928 mL 0.7856 mL 1.9639 mL
80 mM 0.0589 mL 0.2946 mL 0.5892 mL 1.4729 mL
100 mM 0.0471 mL 0.2357 mL 0.4713 mL 1.1784 mL
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N3-C2-NHS ester Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

N3-C2-NHS ester850180-76-6ADC LinkerAntibody-drug conjugates linkerInhibitorinhibitorinhibit

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