2. Metabolic Enzyme/Protease
  3. Endogenous Metabolite
  4. N4-Acetylcytidine triphosphate

N4-Acetylcytidine triphosphate  (Synonyms: ac4CTP)

Cat. No.: HY-111815
Handling Instructions

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N4-Acetylcytidine triphosphate is efficiently used as a substrate in T7 Polymerase-catalyzed in vitro transcription and can be incorporated into multiple templates.

The free form of the compound is prone to instability, it is advisable to consider the stable salt form (N4-Acetylcytidine triphosphate sodium) that retains the same biological activity.

For research use only. We do not sell to patients.

N4-Acetylcytidine triphosphate Chemical Structure

N4-Acetylcytidine triphosphate Chemical Structure

CAS No. : 1428903-57-4

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Other Forms of N4-Acetylcytidine triphosphate:

N4-Acetylcytidine triphosphate Related Antibodies

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1 Publications Citing Use of MCE N4-Acetylcytidine triphosphate

  • Biological Activity

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Description

N4-Acetylcytidine triphosphate is efficiently used as a substrate in T7 Polymerase-catalyzed in vitro transcription and can be incorporated into multiple templates[1].

IC50 & Target[1]

Human Endogenous Metabolite

 

In Vitro

N4-Acetylcytidine triphosphate (0.1-2 mM; 24-48 h) inhibits BV2 microglia cell grwoth[1].
N4-Acetylcytidine triphosphate (0.3-1 mM; 3-6 h) activates NFκB signaling and upregulates NLRP3 expression[1].
N4-Acetylcytidine triphosphate (0.3-1 mM; 3 h) increases the expression level of HMGB1[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

N4-Acetylcytidine triphosphate Related Antibodies

Cell Viability Assay[1]

Cell Line: BV2 microglia
Concentration: 0.1-2 mM
Incubation Time: 24-48 hours
Result: Significantly inhibited BV2 microglia cell growth util cultured for 48 hours with a concentration of 0.3 mM.

Cell Viability Assay[1]

Cell Line: BV2 microglia
Concentration: 0.3 and 1 mM
Incubation Time: 3 and 6 hours
Result: Increased NLRP3 and NFκB expression at both mRNA and protein levels, activated the NFκB signaling.

Western Blot Analysis[1]

Cell Line: BV2 microglia
Concentration: 0.3 and 1 mM
Incubation Time: 3 hours
Result: Induced HMGB1 expression and it is necessary for NFkB signaling, NLRP3 expression and persistent HMGB1 expression.
Molecular Weight

525.19

Formula

C11H18N3O15P3
CAS No.
SMILES

O[C@@H]([C@H]([C@H](N1C(N=C(C=C1)NC(C)=O)=O)O2)O)[C@H]2COP(O)(OP(OP(O)(O)=O)(O)=O)=O
Structure Classification
Initial Source
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

Please store the product under the recommended conditions in the Certificate of Analysis.
Solvent & Solubility
In Vitro: 

H2O : ≥ 250 mg/mL (476.02 mM)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 1.9041 mL 9.5204 mL 19.0407 mL
5 mM 0.3808 mL 1.9041 mL 3.8081 mL
View the Complete Stock Solution Preparation Table

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
Purity & Documentation
References

Complete Stock Solution Preparation Table

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
H2O 1 mM 1.9041 mL 9.5204 mL 19.0407 mL 47.6018 mL
5 mM 0.3808 mL 1.9041 mL 3.8081 mL 9.5204 mL
10 mM 0.1904 mL 0.9520 mL 1.9041 mL 4.7602 mL
15 mM 0.1269 mL 0.6347 mL 1.2694 mL 3.1735 mL
20 mM 0.0952 mL 0.4760 mL 0.9520 mL 2.3801 mL
25 mM 0.0762 mL 0.3808 mL 0.7616 mL 1.9041 mL
30 mM 0.0635 mL 0.3173 mL 0.6347 mL 1.5867 mL
40 mM 0.0476 mL 0.2380 mL 0.4760 mL 1.1900 mL
50 mM 0.0381 mL 0.1904 mL 0.3808 mL 0.9520 mL
60 mM 0.0317 mL 0.1587 mL 0.3173 mL 0.7934 mL
80 mM 0.0238 mL 0.1190 mL 0.2380 mL 0.5950 mL
100 mM 0.0190 mL 0.0952 mL 0.1904 mL 0.4760 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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N4-Acetylcytidine triphosphate Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

N4-Acetylcytidine triphosphate1428903-57-4ac4CTPEndogenous MetabolitemetaboliteBV2 microgliaHMGB1inflamasomeInhibitorinhibitorinhibit

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