N4-Acetylcytidine triphosphate (Synonyms: ac4CTP)

Cat. No.: HY-111815: FAQs
Data Sheet Handling Instructions

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N4-Acetylcytidine triphosphate is efficiently used as a substrate in T7 Polymerase-catalyzed in vitro transcription and can be incorporated into multiple templates.

The free form of the compound is prone to instability, it is advisable to consider the stable salt form (N4-Acetylcytidine triphosphate sodium) that retains the same biological activity.

For research use only. We do not sell to patients.

N4-Acetylcytidine triphosphate Chemical Structure

CAS No. : 1428903-57-4

Size	Stock
5 mg	Get quote	Estimated Time of Arrival: December 31
10 mg	Get quote	Estimated Time of Arrival: December 31
25 mg	Get quote	Estimated Time of Arrival: December 31

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This product is a controlled substance and not for sale in your territory.

Other Forms of N4-Acetylcytidine triphosphate:

N4-Acetylcytidine triphosphate sodium Get quote
N4-Acetylcytidine triphosphate sodium (solution) Get quote

1 Publications Citing Use of MCE N4-Acetylcytidine triphosphate

•Nucleic Acids Res. 2022 Aug 16;gkac675. [Abstract]

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Biological Activity
Purity & Documentation
References
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Description

N4-Acetylcytidine triphosphate is efficiently used as a substrate in T7 Polymerase-catalyzed in vitro transcription and can be incorporated into multiple templates^[1].

IC₅₀ & Target^[1]

Human Endogenous Metabolite

In Vitro

N4-Acetylcytidine triphosphate (0.1-2 mM; 24-48 h) inhibits BV2 microglia cell grwoth^[1].
N4-Acetylcytidine triphosphate (0.3-1 mM; 3-6 h) activates NFκB signaling and upregulates NLRP3 expression^[1].
N4-Acetylcytidine triphosphate (0.3-1 mM; 3 h) increases the expression level of HMGB1^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay^[1]

Cell Line:	BV2 microglia
Concentration:	0.1-2 mM
Incubation Time:	24-48 hours
Result:	Significantly inhibited BV2 microglia cell growth util cultured for 48 hours with a concentration of 0.3 mM.

Cell Viability Assay^[1]

Cell Line:	BV2 microglia
Concentration:	0.3 and 1 mM
Incubation Time:	3 and 6 hours
Result:	Increased NLRP3 and NFκB expression at both mRNA and protein levels, activated the NFκB signaling.

Western Blot Analysis^[1]

Cell Line:	BV2 microglia
Concentration:	0.3 and 1 mM
Incubation Time:	3 hours
Result:	Induced HMGB1 expression and it is necessary for NFkB signaling, NLRP3 expression and persistent HMGB1 expression.

Molecular Weight

525.19

Formula

C₁₁H₁₈N₃O₁₅P₃

CAS No.

1428903-57-4

SMILES

O[C@@H]([C@H]([C@H](N1C(N=C(C=C1)NC(C)=O)=O)O2)O)[C@H]2COP(O)(OP(OP(O)(O)=O)(O)=O)=O

Structure Classification

Others

Initial Source

Endogenous metabolite

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Please store the product under the recommended conditions in the Certificate of Analysis.

Solvent & Solubility

In Vitro:

H₂O : ≥ 250 mg/mL (476.02 mM)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	1.9041 mL	9.5204 mL	19.0407 mL
5 mM	0.3808 mL	1.9041 mL	3.8081 mL
10 mM	0.1904 mL	0.9520 mL	1.9041 mL

View the Complete Stock Solution Preparation Table

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

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In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).

Purity & Documentation

Handling Instructions (2659 KB)

References

[1]. Duan J, et al. N4-acetylcytidine is required for sustained NLRP3 inflammasome activation via HMGB1 pathway in microglia. Cell Signal. 2019 Mar 7;58:44-52. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

H₂O	1 mM	1.9041 mL	9.5204 mL	19.0407 mL	47.6018 mL
	5 mM	0.3808 mL	1.9041 mL	3.8081 mL	9.5204 mL
	10 mM	0.1904 mL	0.9520 mL	1.9041 mL	4.7602 mL
	15 mM	0.1269 mL	0.6347 mL	1.2694 mL	3.1735 mL
	20 mM	0.0952 mL	0.4760 mL	0.9520 mL	2.3801 mL
	25 mM	0.0762 mL	0.3808 mL	0.7616 mL	1.9041 mL
	30 mM	0.0635 mL	0.3173 mL	0.6347 mL	1.5867 mL
	40 mM	0.0476 mL	0.2380 mL	0.4760 mL	1.1900 mL
	50 mM	0.0381 mL	0.1904 mL	0.3808 mL	0.9520 mL
	60 mM	0.0317 mL	0.1587 mL	0.3173 mL	0.7934 mL
	80 mM	0.0238 mL	0.1190 mL	0.2380 mL	0.5950 mL
	100 mM	0.0190 mL	0.0952 mL	0.1904 mL	0.4760 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

N4-Acetylcytidine triphosphate Related Classifications

Metabolic Disease

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

N4-Acetylcytidine triphosphate1428903-57-4ac4CTPEndogenous MetabolitemetaboliteBV2 microgliaHMGB1inflamasomeInhibitorinhibitorinhibit

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