Nek2/Hec1-IN-2

Cat. No.: HY-153749 Purity: ≥98.0%: FAQs
Data Sheet SDS COA Handling Instructions

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Nek2/Hec1-IN-2 (Compound 14) is an inhibitor of Nek2/Hec1. Nek2/Hec1-IN-2 inhibits cancer cell proliferation with IC₅₀ >25 μM.

For research use only. We do not sell to patients.

Nek2/Hec1-IN-2 Chemical Structure

CAS No. : 544416-00-4

Size	Price	Stock	Quantity
5 mg	USD 50	In-stock	Estimated Time of Arrival: December 31
10 mg	USD 80	In-stock	Estimated Time of Arrival: December 31
50 mg		Get quote
100 mg		Get quote

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Based on 1 publication(s) in Google Scholar

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Biological Activity
Purity & Documentation
References
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Description

Nek2/Hec1-IN-2 (Compound 14) is an inhibitor of Nek2/Hec1. Nek2/Hec1-IN-2 inhibits cancer cell proliferation with IC₅₀ >25 μM^[1].

IC₅₀ & Target

NEK2

Cellular Effect

Cell Line	Type	Value	Description	References
HeLa	IC₅₀	> 25 μM Compound: 14	Cytotoxicity against human HeLa cells after 4 days by XTT assay Cytotoxicity against human HeLa cells after 4 days by XTT assay	[PMID: 19243176]
K562	IC₅₀	> 25 μM Compound: 14	Cytotoxicity against human K562 cells after 4 days by XTT assay Cytotoxicity against human K562 cells after 4 days by XTT assay	[PMID: 19243176]
MDA-MB-231	IC₅₀	> 25 μM Compound: 14	Cytotoxicity against human MDA-MB-231 cells after 4 days XTT assay Cytotoxicity against human MDA-MB-231 cells after 4 days XTT assay	[PMID: 19243176]
MDA-MB-468	IC₅₀	> 25 μM Compound: 14	Cytotoxicity against human MDA-MB-468 cells after 4 days by XTT assay Cytotoxicity against human MDA-MB-468 cells after 4 days by XTT assay	[PMID: 19243176]

Molecular Weight

310.37

Formula

C₁₆H₁₄N₄OS

CAS No.

544416-00-4

Appearance

Solid

Color

Off-white to light yellow

SMILES

O=C(C1=NC=CN=C1)NC2=NC(C3=C(C)C=C(C)C=C3)=CS2

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvent & Solubility

In Vitro:

DMSO : 10 mg/mL (32.22 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	3.2220 mL	16.1098 mL	32.2196 mL
5 mM	0.6444 mL	3.2220 mL	6.4439 mL
10 mM	0.3222 mL	1.6110 mL	3.2220 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

Purity & Documentation

Purity: ≥98.0%

Select Batch:

Data Sheet (269 KB) SDS (252 KB)

COA (249 KB) HNMR (109 KB)

Handling Instructions (2659 KB)

References

[1]. Qiu XL, et al. Synthesis and biological evaluation of a series of novel inhibitor of Nek2/Hec1 analogues. J Med Chem. 2009 Mar 26;52(6):1757-67. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	3.2220 mL	16.1098 mL	32.2196 mL	80.5490 mL
	5 mM	0.6444 mL	3.2220 mL	6.4439 mL	16.1098 mL
	10 mM	0.3222 mL	1.6110 mL	3.2220 mL	8.0549 mL
	15 mM	0.2148 mL	1.0740 mL	2.1480 mL	5.3699 mL
	20 mM	0.1611 mL	0.8055 mL	1.6110 mL	4.0275 mL
	25 mM	0.1289 mL	0.6444 mL	1.2888 mL	3.2220 mL
	30 mM	0.1074 mL	0.5370 mL	1.0740 mL	2.6850 mL

Nek2/Hec1-IN-2 Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Nek2/Hec1-IN-2544416-00-4NEKsNIMA–related KinasesNIMA (never in mitosis gene a)-related Expressed KinasesBreast cancerNek2Hec1Inhibitorinhibitorinhibit

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Nek2/Hec1-IN-2

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Nek2/Hec1-IN-2 Related Antibodies

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•Related Screening Libraries:

•Related Small Molecules:

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View All NEKs Isoform Specific Products:

Biological Activity

Purity & Documentation

References

Customer Review

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Complete Stock Solution Preparation Table

Nek2/Hec1-IN-2 Related Classifications

Keywords:

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