NLRP3-IN-32 

NLRP3-IN-32 (compound 7a), a 3, 4-dihydronaphthalene-1(2H)-one derivative, is a potential NLRP3 inflammatory vesicles inhibitor. NLRP3-IN-32 can block the assembly and activation of NLRP3 inflammasome by down-regulating the expression of NLPR3 and apoptosis-associated speck-like protein containing a CARD (ASC), and inhibiting the production of reactive oxygen species (ROS) and other inflammatory mediators. NLRP3-IN-32 inhibits the phosphorylation of IκBα and NF-κB/p65 and the nuclear translocation of p65, thereby inhibiting NF-κB signaling^[1].

NLRP3-IN-32 (compound 7a; 1.5, 3, 6 μM) can reverse the release of macrophage inflammatory factors TNF-α, IL-6, IL-18 and IL-1β under LPS (HY-D1056) stimulation in a dose-dependent manner in RAW246.7 cells^[1].
NLRP3-IN-32 (1.5, 3, 6 μM) inhibits the activation of NLRP3 inflammatory vesicles by dose-dependently scavenging intracellular ROS and NO production by LPS^[1].
NLRP3-IN-32 (1.5, 3, 6 μM) has low toxicity to RAW246.7 cells and has the apoptosis rates of 3.8%, 5.6%, and 6.8%, respectively^[1].
NLRP3-IN-32 (1.5, 3, 6 μM; 24 h) shows the dose-dependent inhibition on the phosphorylation of IκBα and p65 phosphorylation levels stimulated with LPS (1.0 μg/mL; 2h) to inhibit NF-κB signaling pathway activation^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

412.32

C₂₁H₂₂BrN₃O

O=C(C1=C(CC/2)C=CC(Br)=C1)C2=C\C3=NC=C(N4CCN(C)CC4)C=C3

Room temperature in continental US; may vary elsewhere.

Please store the product under the recommended conditions in the Certificate of Analysis.

• [1]. Wen-Xuan Li, et al. Discovery of anti-inflammatory agents from 3, 4-dihydronaphthalene-1(2H)-one derivatives by inhibiting NLRP3 inflammasome activation. Eur J Med Chem. 2024 Feb 29:268:116284.  [Content Brief]