NSP-SA-NHS 

NSP-SA-NHS is an acridinium ester that can be used for chemiluminescent immunoassay. A rapid and sensitive chemiluminescent immunoassay of Bisphenol A (BPA) with NSP-SA-NHS-labeled has been developed^[1].

NSP-SA-NHS marking^[1]
(1) 1mg BSA was dissolved in 0.3 mL PBS (solution A), and 0.2 mg NSP-SA-NHS was dissolved in 40 μL DMF (solution B).
(2) Add solution B to solution A, mix at 18°C, 130 rpm, and dark for 12 h.
(3) The mixture was dialyzed with 500 mL of distilled water at 4°C for 2 d, during which distilled water was changed twice (solution C).
(4) Slowly add solution B to solution C, mix at 18°C, 130 rpm, and dark for 24 h, and then dialysis with distilled water for 48 h. During this period, change distilled water four times.
(5) Dilute the coupling compound with distilled water to a suitable concentration, and analyze it with an ultraviolet spectrophotometer.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

681.73

C₃₂H₃₁N₃O₁₀S₂

199293-83-9

Solid

Light yellow to yellow

O=S(CCC[N+]1=C2C=CC=CC2=C(C(N(CCCC(ON3C(CCC3=O)=O)=O)S(=O)(C4=CC=C(C)C=C4)=O)=O)C5=C1C=CC=C5)([O-])=O

Room temperature in continental US; may vary elsewhere.

-20°C, sealed storage, away from moisture and light

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

• [1]. Fan Fan Yang, et al. A Rapid and Sensitive Chemiluminescent Immunoassay of Bisphenol a with NSP-SA-NHS-Labeled. Applied Mechanics & Materials, 2014, 707:7-11.