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  4. Nuclear Fast Red

Nuclear Fast Red  (Synonyms: Kernechtrot)

Cat. No.: HY-D0943 Purity: 97.48%
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Nuclear Fast Red, an anthraquinone dye, is commonly used in conjunction with an excess of aluminum ions as a red nuclear counterstain. Nuclear fast red has been used as a histochemical and colorimetric reagent for calcium. Nuclear fast red as highly sensitive "off/on" fluorescent probe for detecting guanine.

For research use only. We do not sell to patients.

Nuclear Fast Red Chemical Structure

Nuclear Fast Red Chemical Structure

CAS No. : 6409-77-4

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Nuclear Fast Red Related Antibodies

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Description

Nuclear Fast Red, an anthraquinone dye, is commonly used in conjunction with an excess of aluminum ions as a red nuclear counterstain. Nuclear fast red has been used as a histochemical and colorimetric reagent for calcium[1]. Nuclear fast red as highly sensitive "off/on" fluorescent probe for detecting guanine[2].

Molecular Weight

357.27

Formula

C14H8NNaO7S
CAS No.
Appearance

Solid

Color

Brown to black

SMILES

O=S(C(C(O)=C1C2=O)=C(O)C(N)=C1C(C3=C2C=CC=C3)=O)(O[Na])=O
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

-20°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvent & Solubility
In Vitro: 

DMSO : 5.83 mg/mL (16.32 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.7990 mL 13.9950 mL 27.9900 mL
5 mM 0.5598 mL 2.7990 mL 5.5980 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 2.7990 mL 13.9950 mL 27.9900 mL 69.9751 mL
5 mM 0.5598 mL 2.7990 mL 5.5980 mL 13.9950 mL
10 mM 0.2799 mL 1.3995 mL 2.7990 mL 6.9975 mL
15 mM 0.1866 mL 0.9330 mL 1.8660 mL 4.6650 mL
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Nuclear Fast Red Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Nuclear Fast Red6409-77-4KernechtrotFluorescent Dyeanthraquinone dyehistochemicalcolorimetric reagentcalciumguanineInhibitorinhibitorinhibit

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