Nuclear Fast Red  (Synonyms: Kernechtrot)

Nuclear Fast Red, an anthraquinone dye, is commonly used in conjunction with an excess of aluminum ions as a red nuclear counterstain. Nuclear fast red has been used as a histochemical and colorimetric reagent for calcium^[1]. Nuclear fast red as highly sensitive "off/on" fluorescent probe for detecting guanine^[2].

357.27

C₁₄H₈NNaO₇S

6409-77-4

Solid

Brown to black

O=S(C(C(O)=C1C2=O)=C(O)C(N)=C1C(C3=C2C=CC=C3)=O)(O[Na])=O

Room temperature in continental US; may vary elsewhere.

-20°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

• [1]. Yi Cui, et al. Nuclear fast red as highly sensitive "off/on" fluorescent probe for detecting guanine. Talanta. 2014 Dec;130:536-41.  [Content Brief]

  [2]. M Frank , et al. Certification procedures for nuclear fast red (Kernechtrot), CI 60760. Biotech Histochem. 2007 Feb;82(1):35-9.  [Content Brief]