2. Induced Disease Models Products Metabolic Enzyme/Protease
  3. Cancer Models Nervous System Disease Models Phosphatase
  4. Skin Cancer Models Alzheimer's Disease Models
  5. Okadaic acid ammonium salt

Okadaic acid ammonium salt, a marine toxin, is an inhibitor of protein phosphatases (PP). Okadaic acid ammonium salt has a significantly higher affinity for PP2A (IC50=0.1-0.3 nM), and inhibits PP1 (IC50=15-50 nM), PP3 (IC50=3.7-4 nM), PP4 (IC50=0.1 nM), PP5 (IC50=3.5 nM), but does not inhibit PP2C. Okadaic acid ammonium salt increases of phosphorylation of a number of proteins by inhibiting PP, and acts as a tumor promoter. Okadaic acid ammonium salt induces tau phosphorylation.

For research use only. We do not sell to patients.

Okadaic acid ammonium salt Chemical Structure

Okadaic acid ammonium salt Chemical Structure

CAS No. : 175522-42-6

Size Stock
50 mg   Get quote  
100 mg   Get quote  
250 mg   Get quote  
Synthetic products have potential research and development risk.

* Please select Quantity before adding items.

This product is a controlled substance and not for sale in your territory.

Other Forms of Okadaic acid ammonium salt:

Okadaic acid ammonium salt Related Antibodies

Top Publications Citing Use of Products

  • Biological Activity

  • Purity & Documentation

  • References

  • Customer Review

Description

Okadaic acid ammonium salt, a marine toxin, is an inhibitor of protein phosphatases (PP). Okadaic acid ammonium salt has a significantly higher affinity for PP2A (IC50=0.1-0.3 nM), and inhibits PP1 (IC50=15-50 nM), PP3 (IC50=3.7-4 nM), PP4 (IC50=0.1 nM), PP5 (IC50=3.5 nM), but does not inhibit PP2C. Okadaic acid ammonium salt increases of phosphorylation of a number of proteins by inhibiting PP, and acts as a tumor promoter. Okadaic acid ammonium salt induces tau phosphorylation[1][2].

IC50 & Target[1]

PP1

15-50 nM (IC50)

PP2A

0.1-0.3 nM (IC50)

PP3

3.7-4 nM (IC50)

PP4

0.1 nM (IC50)

PP5

3.5 nM (IC50)

PP2B

~4000 nM (IC50)

PP7

＞1000 nM (IC50)

In Vitro

Okadaic acid ammonium salt (0-100 nM; 24 h or 48 h) inhibits the proliferation of AGS, MNK-45, Caco 2 cells[3].
Okadaic acid (10 nM, 8 hours) ammonium salt increases Drp1 phosphorylation and mitochondrial fission in rat cortical neurons[4].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Okadaic acid ammonium salt Related Antibodies

Cell Proliferation Assay[3]

Cell Line: AGS, MNK-45 and Caco 2 cell lines
Concentration: 0-100 nM
Incubation Time: 24 h or 48 h
Result: Inhibited the proliferation of AGS, MNK-45, Caco 2 cells.
In Vivo

Okadaic acid ammonium salt can be used in animal modeling to create Alzheimer's disease and skin tumor models[6][7][8].

1. Induction of neurotoxicity[6][7]
Background
Tau protein hyperphosphorylation is a major pathological hallmark of Alzheimer’s disease (AD). Okadaic acid can induce a decrease in protein phosphatase (PP-2A) activity, leading to tau protein hyperphosphorylation[6].
Specific Mmodeling Methods
Rat: Wistar • male • 2-month-old
Administration: 200 ng • icv • single dose
Note
(1) Dissolve 200 ng of Okadaic acid in artificial cerebrospinal fluid (aCSF), which contains: 147 mmol/L NaCl, 2.9 mmol/L KCl, 1.6 mmol/L MgCl2, 1.7 mmol/L CaCl2, and 2.2 mmol/L glucose. The injection volume of aCSF is 10 μL per rat.
(2) After intracerebroventricular injection of Okadaic acid, administer intraperitoneal gentamicin (5 mg/kg) to prevent sepsis.
(3) Combined treatment with hypoxia can enhance the neurotoxic effects induced by Okadaic acid. Hypoxia treatment method: After intracerebroventricular injection of Okadaic acid, place the rats in a hypoxic chamber with 10% oxygen for 22 hours/day for 3 consecutive days.
Modeling Indicators
Molecular changes: Reduced levels of procaspase 3, decreased AChE activity and expression, increased levels of caspase 3, β-amyloid protein (Aβ42), and phosphorylated tau protein, and accumulation of ROS and malondialdehyde (MDA).
Phenotypic observations: Neuronal damage and death in the rat brain, with cell swelling and nuclear chromatin condensation.
Correlated Product(s): /
Opposite Product(s): /

2. Induction of skin tumors[8]
Background
Okadaic acid is a skin irritant and toxic polyether compound that acts as a non-TPA-type tumor promoter. Okadaic acid stimulates ornithine decarboxylase in mouse ears and mouse skin, but does not induce adhesion of human promyelocytic leukemia cells (HL-60 cells), does not inhibit the specific binding of [3H]TPA to mouse particle components, and does not activate protein kinase C[8].
Specific Mmodeling Methods
Mice: CD-1 • female • 8-week-old
Administration: 10 μg • topical application • twice a week for 30 weeks
Note
(1) Dissolve 10 μg of Okadaic acid in acetone and apply a volume of 0.1 mL per rat.
(2) The induction of skin tumors can be divided into two stages, with local application of Okadaic acid being the second stage. The first stage involves local application of DMBA dissolved in acetone (100 μg DMBA in 0.1 mL acetone), followed by the second stage one week later. Both Okadaic acid alone and the combination of Okadaic acid and DMBA induce skin tumors, with the combined treatment showing enhanced induction effects.
Modeling Indicators
Phenotypic observations: Increased incidence of papillomas and sarcomas on the skin surface.
Correlated Product(s): DMBA (HY-W011845); Teleocidin
Opposite Product(s): /

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Female wild-type C57BL/6 mice (6 to 8 months)[5]
Dosage: 100 μM
Administration: Injected unilaterally to the lateral amygdala
Result: Induced Tau phosphorylation and protein aggregation in anatomically distinct brain regions 24 h post-injection.
Molecular Weight

822.03

Formula

C44H71NO13
CAS No.
SMILES

O[C@H](CC1)[C@@](O[C@@H]1C[C@@](C)(O)C([O-])=O)(C=C2C)O[C@](C2)([H])[C@H](C)/C=C/[C@@H]3O[C@@]4(O[C@]([C@@H](C5=C)O)([H])[C@@](O[C@]5([H])[C@@H](O)C[C@@H]([C@@]6([H])O[C@@]7(CCCCO7)CC[C@H]6C)C)([H])CC4)CC3.[NH4+]
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

Please store the product under the recommended conditions in the Certificate of Analysis.
Purity & Documentation
References
  • No file chosen (Maximum size is: 1024 Kb)
  • If you have published this work, please enter the PubMed ID.
  • Your name will appear on the site.

Okadaic acid ammonium salt Related Classifications

  • Molarity Calculator

  • Dilution Calculator

The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass   Concentration   Volume   Molecular Weight *
= × ×

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Okadaic acid175522-42-6Phosphatasemarine toxinAGSMNK-45Caco 2 cellsphosphorylationtumor promoterDrp1Inhibitorinhibitorinhibit

Your Recently Viewed Products:

Inquiry Online

Your information is safe with us. * Required Fields.

Product Name

  

Salutation

Applicant Name *

 

Email Address *

Phone Number *

 

Organization Name *

Department *

 

Requested quantity *

Country or Region *

      

Remarks

Bulk Inquiry

Inquiry Information

Product Name:
Okadaic acid ammonium salt
Cat. No.:
HY-115760
Quantity:
MCE Japan Authorized Agent:

Customer Review

Thank You for Your Feedback!

Request for HNMR Report

Please fill out this form to request the QC report. We will send it to your Email address shortly.

Your information is safe with us. * Required Fields.

Product Name

  

Lot #

Applicant Name *

 

Email Address *

Phone Number

 

Department *

Organization Name *

 

Country or Region *

Remarks

SAFETY DATA SHEET (SDS)

English - EN (0 KB) Français - FR (0 KB) Deutsch - DE (0 KB) Norwegian - NO (0 KB) Español - ES (0 KB) Swedish - SV (0 KB)

Request for HNMR Report

We have received your request and will respond to you as soon as possible.