Okadaic acid

Name: Okadaic acid
Brand: MedChemExpress
SKU: HY-N6785
Rating: 5.0 (11 reviews)

Cat. No.: HY-N6785 Purity: 99.19%: FAQs
Data Sheet SDS COA Handling Instructions

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Okadaic acid, a marine toxin, is an inhibitor of protein phosphatases (PP). Okadaic acid has a significantly higher affinity for PP2A (IC₅₀=0.1-0.3 nM), and inhibits PP1 (IC₅₀=15-50 nM), PP3 (IC₅₀=3.7-4 nM), PP4 (IC₅₀=0.1 nM), PP5 (IC₅₀=3.5 nM), but does not inhibit PP2C. Okadaic acid increases of phosphorylation of a number of proteins by inhibiting PP, and acts a tumor promoter. Okadaic acid induces tau phosphorylation.

For research use only. We do not sell to patients.

Okadaic acid Chemical Structure

CAS No. : 78111-17-8

Size	Price	Stock	Quantity
Solvent
25 μg (124.2 μM * 250 μL in Ethanol)	USD 230	In-stock	Estimated Time of Arrival: December 31

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Based on 11 publication(s) in Google Scholar

Other Forms of Okadaic acid:

11 Publications Citing Use of MCE Okadaic acid

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Biological Activity
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Description

IC₅₀ & Target^[1]

PP1

15-50 nM (IC₅₀)

PP2A

0.1-0.3 nM (IC₅₀)

PP3

3.7-4 nM (IC₅₀)

PP4

0.1 nM (IC₅₀)

PP5

3.5 nM (IC₅₀)

PP2B

~4000 nM (IC₅₀)

PP7

＞1000 nM (IC₅₀)

Cellular Effect

Cell Line	Type	Value	Description	References
HCT-116	IC₅₀	0.67 μM Compound: OA	Cytotoxicity against human HCT116 cells after 24 hrs by MTT assay Cytotoxicity against human HCT116 cells after 24 hrs by MTT assay	[PMID: 28383915]
HepG2	IC₅₀	0.54 μM Compound: OA	Cytotoxicity against human HepG2 cells after 24 hrs by MTT assay Cytotoxicity against human HepG2 cells after 24 hrs by MTT assay	[PMID: 28383915]
N2a	IC₅₀	0.85 μM Compound: OA	Cytotoxicity against mouse Neuro2a cells after 24 hrs by MTT assay Cytotoxicity against mouse Neuro2a cells after 24 hrs by MTT assay	[PMID: 28383915]
P388	EC₅₀	87 nM Compound: OA	Blockade of okadaic acid-induced cytotoxicity in mouse P388 cells after 2 days by WST-8 assay in presence of Halichondria okadai recombinant OABP 2.3 Blockade of okadaic acid-induced cytotoxicity in mouse P388 cells after 2 days by WST-8 assay in presence of Halichondria okadai recombinant OABP 2.3	[PMID: 20863709]
P388	EC₅₀	92 nM Compound: OA	Blockade of okadaic acid-induced cytotoxicity in mouse P388 cells after 2 days by WST-8 assay in presence of Halichondria okadai recombinant OABP 2.1 Blockade of okadaic acid-induced cytotoxicity in mouse P388 cells after 2 days by WST-8 assay in presence of Halichondria okadai recombinant OABP 2.1	[PMID: 20863709]

In Vitro

Okadaic acid (0-100 nM; 24 h or 48 h) inhibits the proliferation of AGS, MNK-45, Caco 2 cells^[3].
Okadaic acid (10 nM; 8 hours) increases Drp1 phosphorylation and mitochondrial fission in rat cortical neurons^[4].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Proliferation Assay^[3]

Cell Line:	AGS, MNK-45 and Caco 2 cell lines
Concentration:	0-100 nM
Incubation Time:	24 h or 48 h
Result:	Inhibited the proliferation of AGS, MNK-45, Caco 2 cells.

In Vivo

Okadaic acid can be used to induce Alzheimer's disease models^[6].

Induction of Alzheimer's disease model^[6]

Background

Okadaic acid is a Ser/Thr phosphatase enzyme inhibitor that causes Tau hyperphosphorylation and induces the intracellular aggregation of neurofibrillary tangles.

Specific Mmodeling Methods

Mice: Wistar • male •-250–300 g
Administration: 100μg • icv • single dose

Note

Modeling Indicators

Molecular changes: Induced increases in pTau^ser396 in the hippocampus.

Correlated Product(s): /

Opposite Product(s): /

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

805.00

Formula

C₄₄H₆₈O₁₃

CAS No.

78111-17-8

Appearance

Liquid

Color

Colorless to light yellow

SMILES

O[C@H](CC[C@@H](C[C@@](C)(O)C(O)=O)O1)[C@@]1(C=C(C)C2)O[C@]2([H])[C@H](C)/C=C/[C@@H]3O[C@@]4(O[C@]([C@@H]5O)([H])[C@@](O[C@@]([C@@H](O)C[C@@H]([C@@]6([H])O[C@@]7(CCCCO7)CC[C@H]6C)C)([H])C5=C)([H])CC4)CC3

Structure Classification

Ketones, Aldehydes, Acids

Initial Source

Animals

bivalve mollusks

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Solution, -20°C, 2 years

Purity & Documentation

Purity: 99.19%

Select Batch:

Data Sheet (277 KB) SDS (480 KB)

COA (249 KB) RP-HPLC (341 KB)

Handling Instructions (2659 KB)

References

[1]. Kleppe R, et al. Cell Death Inducing Microbial Protein Phosphatase Inhibitors--Mechanisms of Action. Mar Drugs. 2015 Oct 22;13(10):6505-20. [Content Brief]

[2]. Valdiglesias V, et al. Okadaic acid: more than a diarrheic toxin. Mar Drugs. 2013 Oct 31;11(11):4328-49. [Content Brief]

[3]. del Campo M, et al. Okadaic acid toxin at sublethal dose produced cell proliferation in gastric and colon epithelial cell lines. Mar Drugs. 2013;11(12):4751-4760. [Content Brief]

[4]. Cho MH, et al. Increased phosphorylation of dynamin-related protein 1 and mitochondrial fission in okadaic acid-treated neurons. Brain Res. 2012 May 15;1454:100-10. [Content Brief]

[5]. Baker S, et al. A local insult of okadaic acid in wild-type mice induces tau phosphorylation and protein aggregation in anatomically distinct brain regions. Acta Neuropathol Commun. 2016;4:32. [Content Brief]

[6]. Natalia Dos Santos Tramontin, et al. Gold Nanoparticles Treatment Reverses Brain Damage in Alzheimer's Disease Model . Mol Neurobiol. 2020, 57, 2. [Content Brief]

Okadaic acid Related Classifications

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Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Okadaic acid78111-17-8PhosphataseApoptosisHalichondriaserine/threonine-specificproteinphosphatasesPP1PP2APP3Inhibitorinhibitorinhibit

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Okadaic acid

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11 Publications Citing Use of MCE Okadaic acid

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