Osteostatin (Synonyms: PTHrP (107–111))

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Osteostatin, a fragment of parathyroid hormone-related protein (PTHrP) 107-111, promotes bone repair in animal models of bone defects and prevents bone erosion in inflammatory arthritis, inhibits collagen-induced arthritis and inhibits osteoclastic bone resorption directly. Osteostatin can be used for inflammation and immunology research.

For research use only. We do not sell to patients.

Osteostatin Chemical Structure

CAS No. : 138949-73-2

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Description

In Vitro

Osteostatin (100, 250 and 500 nM, 7-9 days) decreases the differentiation of osteoclasts in a concentration-dependent manner^[2].
Osteostatin (100, 250 and 500 nM, 7 days) decreases the mRNA levels of cathepsin K, osteoclast associated Ig-like receptor (OSCAR) and NFATc1^[2].
Osteostatin (100, 250 and 500 nM, 2 days) inhibits the nuclear translocation of the master transcription factor NFATc1 in osteoclast differentiation ^[2].
Osteostatin (0.1-100 nM, 2 days) significantly increased cell growth in MC3T3-E1 cells^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Differentiation Assay^[2]

Cell Line:	PBMCs are cultured with M-CSF and RANKL
Concentration:	100, 250 and 500 nM
Incubation Time:	7-9 days
Result:	Decreased the differentiation of osteoclasts in a concentration-dependent manner, but it does not modify the resorptive ability of mature osteoclasts.

RT-PCR^[2]

Cell Line:	PBMCs are cultured with M-CSF and RANKL
Concentration:	100, 250 and 500 nM
Incubation Time:	7 days
Result:	Decreased the mRNA levels of cathepsin K, osteoclast associated Ig-like receptor (OSCAR) and NFATc1.

Immunofluorescence^[2]

Cell Line:	PBMCs are cultured with M-CSF and RANKL
Concentration:	100, 250 and 500 nM
Incubation Time:	2 days
Result:	Inhibited the nuclear translocation of the master transcription factor in osteoclast differentiation NFATc1.

Cell Proliferation Assay^[3]

Cell Line:	MC3T3-E1 cells
Concentration:	0-100 nM
Incubation Time:	2 days
Result:	Significantly increased cell growth of MC3T3-E1 cells

In Vivo

Osteostatin (80 or 120 μg/kg,; after the onset of disease s.c. every day for 13 days) decreases the severity of arthritis as well as cartilage and bone degradation^[4].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	Collagen-induced arthritis in male DBA/1 mice^[4]
Dosage:	80 or 120 μg/kg
Administration:	After the onset of disease s.c. every day for 13 days
Result:	Reduced serum IgG2a levels as well as T cell activation, with the downregulation of RORγt+CD4+ T cells and upregulation of FoxP3+CD8+ T cells in lymph nodes. Decreased the levels of key cytokines, such as interleukin (IL)-1β, IL-2, IL-6, IL-17, and tumor necrosis factor-α in mice paws, whereas enhanced IL-10.

Molecular Weight

619.67

Formula

C₂₇H₄₁N₉O₈

CAS No.

138949-73-2

Sequence

Thr-Arg-Ser-Ala-Trp

Sequence Shortening

TRSAW

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Please store the product under the recommended conditions in the Certificate of Analysis.

Purity & Documentation

Handling Instructions (2659 KB)

References

[1]. Fenton AJ, et.al. A potent inhibitor of osteoclastic bone resorption within a highly conserved pentapeptide region of parathyroid hormone-related protein; PTHrP[107-111]. Endocrinology. 1991 Dec;129(6):3424-6. [Content Brief]

[2]. Ibáñez L, et.al. Osteostatin Inhibits M-CSF+RANKL-Induced Human Osteoclast Differentiation by Modulating NFATc1. Int J Mol Sci. 2022 Aug 1;23(15):8551. [Content Brief]

[3]. Lozano D, et.al. Osteostatin-loaded bioceramics stimulate osteoblastic growth and differentiation. Acta Biomater. 2010 Mar;6(3):797-803. [Content Brief]

[4]. Nácher-Juan J, et.al. Osteostatin Inhibits Collagen-Induced Arthritis by Regulation of Immune Activation, Pro-Inflammatory Cytokines, and Osteoclastogenesis. Int J Mol Sci. 2019 Aug 7;20(16):3845. [Content Brief]

Osteostatin Related Classifications

Inflammation/Immunology

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Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Osteostatin138949-73-2PTHrP (107–111)PTHRParathyroid hormone receptorPBMCsosteoclastic boneNFATc1bone defectsArthritisInhibitorinhibitorinhibit

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