Pacanalotamab  (Synonyms: AMG 420; BI-836909)

Pacanalotamab (AMG 420; BI-836909) is a bispecific T-cell engager (BiTE) targeting to BCMA and CD3E. BCMA refers to B cell maturation antigen, as Pacanalotamab redirecting T cells to BCMA expressing cells on the cell surface. Pacanalotamab conducts T-cell redirected lysis of human multiple myeloma (MM) cell lines^[1].

(scFv-heavy-kappa)-(scFv-heavy-lambda)

Chimeric; Humanized

B cell maturation antigen, BCMA; CD3ɛ^[1]

Pacanalotamab (1 pg/mL-100 ng/mL; 24 h) induces BCMA-dependent tumor cell lysis via T cells activation. And Pacanalotamab induces dose-dependent redirected lysis of human multiple myeloma cell lines with EC₉₀ values ranging from 16-810 pg/mL^[1].
Pacanalotamab (100 ng/mL; 24 h) induces translocation of phosphatidylserine from the inner to the outer leaflet of the plasma membrane, leading to apoptosis in MM.1R cells^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Pacanalotamab (50 μg/kg/day; i.v. or s.c.; for 18 days) exhibits anti-tumor activity in mouse NCI-H929 xenograft model^[1].
Pacanalotamab (5 μg/kg/day; i.v. or s.c.) significant prolongs survival of mouse in mouse orthotopic L-363 xenograft model^[1].
Pacanalotamab (135 μg/kg/day for i.v. or 405 μg/kg/day for s.c.; up to 28 days) is well tolerated, and it decreases plasma cells in the bone marrow in macaque in toxicity studies^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

2251756-52-0

Liquid

Colorless to light yellow

[Pacanalotamab]

Shipping with dry ice.

Please store the product under the recommended conditions in the Certificate of Analysis.

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  (scFv-heavy-kappa)-(scFv-heavy-lambda)

• 
  Immobilized Pacanalotamab can bind Human BCMA Protein (ECD, hFc Tag). The EC₅₀ for this effect is 0.597 ng/mL.
• 
  Immobilized Pacanalotamab can bind Human CD3 epsilon/CD3e Protein (ECD, hFc Tag). The EC₅₀ for this effect is 1.506 ng/mL.

• [1]. Hipp S, et al. A novel BCMA/CD3 bispecific T-cell engager for the treatment of multiple myeloma induces selective lysis in vitro and in vivo. Leukemia. 2017 Aug;31(8):1743-1751.  [Content Brief]