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  3. Pararosaniline hydrochloride

Pararosaniline hydrochloride is a pH-responsive basic dye, as a biological stain to track certain proteins. Pararosaniline hydrochloride has been used in the analysis of SO2 and formaldehyde and staining of bacteria or other organisms.

For research use only. We do not sell to patients.

Pararosaniline hydrochloride Chemical Structure

Pararosaniline hydrochloride Chemical Structure

CAS No. : 569-61-9

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Based on 1 publication(s) in Google Scholar

Other Forms of Pararosaniline hydrochloride:

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Description

Pararosaniline hydrochloride is a pH-responsive basic dye, as a biological stain to track certain proteins. Pararosaniline hydrochloride has been used in the analysis of SO2 and formaldehyde and staining of bacteria or other organisms[1][2].

Molecular Weight

323.82

Formula

C19H18ClN3

CAS No.
Appearance

Solid

Color

Green to black

SMILES

N=C(C=C/1)C=CC1=C(C2=CC=C(N)C=C2)\C3=CC=C(N)C=C3.Cl

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

RT, sealed storage, away from moisture and light

In solvent -80°C 2 years
-20°C 1 year
Solvent & Solubility
In Vitro: 

DMSO : 50 mg/mL (154.41 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H2O : 9.46 mg/mL (29.21 mM; ultrasonic and warming and heat to 60°C)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 3.0881 mL 15.4407 mL 30.8814 mL
5 mM 0.6176 mL 3.0881 mL 6.1763 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
Purity & Documentation

Purity: 98.28%

References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
H2O / DMSO 1 mM 3.0881 mL 15.4407 mL 30.8814 mL 77.2034 mL
5 mM 0.6176 mL 3.0881 mL 6.1763 mL 15.4407 mL
10 mM 0.3088 mL 1.5441 mL 3.0881 mL 7.7203 mL
15 mM 0.2059 mL 1.0294 mL 2.0588 mL 5.1469 mL
20 mM 0.1544 mL 0.7720 mL 1.5441 mL 3.8602 mL
25 mM 0.1235 mL 0.6176 mL 1.2353 mL 3.0881 mL
DMSO 30 mM 0.1029 mL 0.5147 mL 1.0294 mL 2.5734 mL
40 mM 0.0772 mL 0.3860 mL 0.7720 mL 1.9301 mL
50 mM 0.0618 mL 0.3088 mL 0.6176 mL 1.5441 mL
60 mM 0.0515 mL 0.2573 mL 0.5147 mL 1.2867 mL
80 mM 0.0386 mL 0.1930 mL 0.3860 mL 0.9650 mL
100 mM 0.0309 mL 0.1544 mL 0.3088 mL 0.7720 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Pararosaniline hydrochloride
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HY-W127770
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