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Pararosaniline hydrochloride  (Synonyms: Basic red 9)

Cat. No.: HY-W127770 Purity: 97.33%
SDS
COA
Handling Instructions Technical Support

Pararosaniline hydrochloride (Basic red 9) is a pH-responsive basic dye, as a biological stain to track certain proteins. The pH of the acidified Pararosaniline hydrochloride reagent has a significant effect on the color and the maximum absorption wavelength (λmax) of the reaction system, with its optimum pH 0.48 and a λmax at 549 nm. Pararosaniline hydrochloride is also a strong modifier of RNA splicing. Pararosaniline hydrochloride has been used in the analysis of SO2 and formaldehyde and staining of bacteria or other organisms. Pararosaniline hydrochloride is extensively used in industries like textile, printing, paper, cosmetic, and leather.

For research use only. We do not sell to patients.

Pararosaniline hydrochloride Chemical Structure

Pararosaniline hydrochloride Chemical Structure

CAS No. : 569-61-9

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Based on 1 publication(s) in Google Scholar

Other Forms of Pararosaniline hydrochloride:

Pararosaniline hydrochloride Related Antibodies

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Description

Pararosaniline hydrochloride (Basic red 9) is a pH-responsive basic dye, as a biological stain to track certain proteins. The pH of the acidified Pararosaniline hydrochloride reagent has a significant effect on the color and the maximum absorption wavelength (λmax) of the reaction system, with its optimum pH 0.48 and a λmax at 549 nm. Pararosaniline hydrochloride is also a strong modifier of RNA splicing. Pararosaniline hydrochloride has been used in the analysis of SO2 and formaldehyde and staining of bacteria or other organisms. Pararosaniline hydrochloride is extensively used in industries like textile, printing, paper, cosmetic, and leather[1][2][3][4][5].

In Vivo

Pararosaniline hydrochloride (0-2 mM, 25 days) induces a decrease in the spliced-in variant of the ret-1 transcript in C. elegans[3].
Pararosaniline hydrochloride (1 mM, 24 h) is deleterious to C. elegans physiology and causes the upregulation of the rbm-17 gene[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Molecular Weight

323.83

Formula

C19H18ClN3
CAS No.
Appearance

Solid

Color

Green to black

SMILES

N=C(C=C/1)C=CC1=C(C2=CC=C(N)C=C2)\C3=CC=C(N)C=C3.Cl
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

RT, sealed storage, away from moisture and light

In solvent -80°C 2 years
-20°C 1 year
Solvent & Solubility
In Vitro: 

DMSO : 50 mg/mL (154.40 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H2O : 9.46 mg/mL (29.21 mM; ultrasonic and warming and heat to 60°C)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 3.0880 mL 15.4402 mL 30.8804 mL
5 mM 0.6176 mL 3.0880 mL 6.1761 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
Purity & Documentation

Purity: 98.28%

SDS (479 KB)

COA (251 KB) HNMR (254 KB) LCMS (88 KB)

Handling Instructions (2659 KB)
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
H2O / DMSO 1 mM 3.0880 mL 15.4402 mL 30.8804 mL 77.2010 mL
5 mM 0.6176 mL 3.0880 mL 6.1761 mL 15.4402 mL
10 mM 0.3088 mL 1.5440 mL 3.0880 mL 7.7201 mL
15 mM 0.2059 mL 1.0293 mL 2.0587 mL 5.1467 mL
20 mM 0.1544 mL 0.7720 mL 1.5440 mL 3.8601 mL
25 mM 0.1235 mL 0.6176 mL 1.2352 mL 3.0880 mL
DMSO 30 mM 0.1029 mL 0.5147 mL 1.0293 mL 2.5734 mL
40 mM 0.0772 mL 0.3860 mL 0.7720 mL 1.9300 mL
50 mM 0.0618 mL 0.3088 mL 0.6176 mL 1.5440 mL
60 mM 0.0515 mL 0.2573 mL 0.5147 mL 1.2867 mL
80 mM 0.0386 mL 0.1930 mL 0.3860 mL 0.9650 mL
100 mM 0.0309 mL 0.1544 mL 0.3088 mL 0.7720 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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Pararosaniline hydrochloride Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Pararosaniline569-61-9Basic red 9Basic red9Basic red-9Fluorescent Dyestaintrack proteinSO2formaldehydeInhibitorinhibitorinhibit

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