2. TGF-beta/Smad
  3. TGF-β Receptor
  4. Pentabromopseudilin

Pentabromopseudilin (PBrP) is a marine antibiotic isolated from the marine bacteria Pseudomonas bromoutilis and Alteromonas luteoviolaceus. PBrP exhibits antimicrobial, anti-tumour and phytotoxic activities. PBrP is a reversible and allosteric inhibitor of myosin Va (MyoVa). PBrP also is a potent inhibitor of transforming growth factor-β (TGF-β) activity. PBrP can be used for the research of fibrotic diseases and cancer.

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Pentabromopseudilin Chemical Structure

Pentabromopseudilin Chemical Structure

CAS No. : 10245-81-5

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Based on 1 publication(s) in Google Scholar

Pentabromopseudilin Related Antibodies

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Description

Pentabromopseudilin (PBrP) is a marine antibiotic isolated from the marine bacteria Pseudomonas bromoutilis and Alteromonas luteoviolaceus. PBrP exhibits antimicrobial, anti-tumour and phytotoxic activities. PBrP is a reversible and allosteric inhibitor of myosin Va (MyoVa). PBrP also is a potent inhibitor of transforming growth factor-β (TGF-β) activity. PBrP can be used for the research of fibrotic diseases and cancer[1].
In Vitro

Pentabromopseudilin (PBrP) (0.01-1 μM, 6 h) prevents TGF-β-induced Smad protein phosphorylation and nuclear translocation[1].
PBrP (0.5 μM, 6 h) inhibits TGF-β-stimulated transcriptional responses[1].
PBrP (0-1 μM, 6 h) inhibits TGF-β-induced EMT in A549 cells[1].
PBrP (0.2 μM, 20 h) suppresses TGF-β-induced cell migration[1].
PBrP (0.01-1 μM, 6 h) blocks TGF-β signalling by enhancing degradation of TβRII[1].
PBrP (0.5 μM, 0, 1, 3 h) blocks TGF-β signalling by enhancing degradation of TβRII via caveolae[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Pentabromopseudilin Related Antibodies

Western Blot Analysis[1]

Cell Line: Mv1Lu, A549, Clone 9 and HepG2 cells
Concentration: 0.01-1 μM (Mv1Lu, A549, Clone 9 and HepG2 cells); 0.5 μM (Mv1Lu, A549 and HepG2 cells)
Incubation Time: 6 h (Mv1Lu, A549, Clone 9 and HepG2 cells); 0.5, 1, 2, 4, 6 h (Mv1Lu, A549 and HepG2 cells)
Result: Suppressed the TGF-β-stimulated Smad2/3 phosphorylation in a dose-dependent manner in these cell lines.
Prevented TGF-β induced the nuclear translocation of Smad2/3 and PBrP alone did not alter the localisation of Smad proteins.

Immunofluorescence[1]

Cell Line: A549 cells
Concentration: 0.2 μM
Incubation Time: 6 h
Result: Abolished TGF-β-induced Smad2/3 nuclear translocation.

Cell Migration Assay [1]

Cell Line: A549 cells
Concentration: 0.2 μM
Incubation Time: 20 h
Result: Suppressed TGF-β-stimulated cell migration and did not close the wound.
Molecular Weight

553.66

Formula

C10H4Br5NO
CAS No.
Appearance

Solid

Color

Off-white to gray

SMILES

OC1=C(C2=C(Br)C(Br)=C(Br)N2)C=C(Br)C=C1Br
Structure Classification
Initial Source

Pseudomonas bromoutilis
Shipping

Room temperature in continental US; may vary elsewhere.
Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
Solvent & Solubility
In Vitro: 

DMSO : ≥ 125 mg/mL (225.77 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 1.8062 mL 9.0308 mL 18.0616 mL
5 mM 0.3612 mL 1.8062 mL 3.6123 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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This equation is commonly abbreviated as: C1V1 = C2V2

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In Vivo Dissolution Calculator
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Purity & Documentation

Purity: 99.63%

SDS (251 KB)

COA (252 KB) HNMR (277 KB) LCMS (98 KB)

Handling Instructions (2659 KB)
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 1.8062 mL 9.0308 mL 18.0616 mL 45.1541 mL
5 mM 0.3612 mL 1.8062 mL 3.6123 mL 9.0308 mL
10 mM 0.1806 mL 0.9031 mL 1.8062 mL 4.5154 mL
15 mM 0.1204 mL 0.6021 mL 1.2041 mL 3.0103 mL
20 mM 0.0903 mL 0.4515 mL 0.9031 mL 2.2577 mL
25 mM 0.0722 mL 0.3612 mL 0.7225 mL 1.8062 mL
30 mM 0.0602 mL 0.3010 mL 0.6021 mL 1.5051 mL
40 mM 0.0452 mL 0.2258 mL 0.4515 mL 1.1289 mL
50 mM 0.0361 mL 0.1806 mL 0.3612 mL 0.9031 mL
60 mM 0.0301 mL 0.1505 mL 0.3010 mL 0.7526 mL
80 mM 0.0226 mL 0.1129 mL 0.2258 mL 0.5644 mL
100 mM 0.0181 mL 0.0903 mL 0.1806 mL 0.4515 mL
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Pentabromopseudilin Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Pentabromopseudilin10245-81-5TGF-β ReceptorTransforming growth factor beta receptorsantibioticPseudomonas bromoutilisAlteromonas luteoviolaceusantimicrobialanti-tumourphytotoxicMyoVaTGF-βfibrotic diseasescancerInhibitorinhibitorinhibit

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