2. Click Chemistry
  3. Alkynes
  4. Propargylcholine bromide

Propargylcholine bromide is a choline analogue containing terminal propargyl that can be incorporated into all classes of Choline-containing phospholipids such as phosphatidylcholine and sphingomyelin, labeling Choline-containing phospholipids. Propargylcholine bromide-labeled phospholipid molecules can be visualized in cells with high sensitivity and spatial resolution. Propargylcholine bromide can be used as a molecular tool to study the biochemical and metabolic processes of Choline-containing phospholipids in cells. Propargylcholine (bromide) is a click chemistry reagent, it contains an Alkyne group and can undergo copper-catalyzed azide-alkyne cycloaddition (CuAAc) with molecules containing Azide groups.

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Propargylcholine bromide Chemical Structure

Propargylcholine bromide Chemical Structure

CAS No. : 111755-76-1

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Based on 1 publication(s) in Google Scholar

Propargylcholine bromide Related Antibodies

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  • Biological Activity

  • Purity & Documentation

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Description

Propargylcholine bromide is a choline analogue containing terminal propargyl that can be incorporated into all classes of Choline-containing phospholipids such as phosphatidylcholine and sphingomyelin, labeling Choline-containing phospholipids. Propargylcholine bromide-labeled phospholipid molecules can be visualized in cells with high sensitivity and spatial resolution. Propargylcholine bromide can be used as a molecular tool to study the biochemical and metabolic processes of Choline-containing phospholipids in cells[1][2]. Propargylcholine (bromide) is a click chemistry reagent, it contains an Alkyne group and can undergo copper-catalyzed azide-alkyne cycloaddition (CuAAc) with molecules containing Azide groups.

In Vitro

Propargylcholine bromide (10, 50 µM; 24 h) incorporates into NIH 3T3 cells and shows strong staining in a concentration-dependent manner (fixed and stained with Alexa568-azide)[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Propargylcholine bromide Related Antibodies

Immunofluorescence[1]

Cell Line: NIH 3T3 cells
Concentration: 10, 50 µM
Incubation Time: 24 h
Result: Showed strong staining proportional in intensity to the concentration of added Propargylcholine bromide.
In Vivo

Propargylcholine bromide (P-Cho; 3.5-4.0 mg/kg; i.p; single daily for 6 days) effectively incorporates into newly synthesized myelin within 1 week of dosing and sustained presence beyond 6 weeks in rhesus monkey[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Molecular Weight

208.10

Formula

C7H14BrNO
CAS No.
Appearance

Solid

Color

Off-white to yellow

SMILES

C#CC[N+](C)(CCO)C.[Br-]
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Solvent & Solubility
In Vitro: 

DMSO : 125 mg/mL (600.67 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H2O : 125 mg/mL (600.67 mM; Need ultrasonic)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 4.8054 mL 24.0269 mL 48.0538 mL
5 mM 0.9611 mL 4.8054 mL 9.6108 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
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Volume
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Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

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Volume (start)

V1

=
Concentration (final)

C2

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Volume (final)

V2

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO / H2O 1 mM 4.8054 mL 24.0269 mL 48.0538 mL 120.1345 mL
5 mM 0.9611 mL 4.8054 mL 9.6108 mL 24.0269 mL
10 mM 0.4805 mL 2.4027 mL 4.8054 mL 12.0135 mL
15 mM 0.3204 mL 1.6018 mL 3.2036 mL 8.0090 mL
20 mM 0.2403 mL 1.2013 mL 2.4027 mL 6.0067 mL
25 mM 0.1922 mL 0.9611 mL 1.9222 mL 4.8054 mL
30 mM 0.1602 mL 0.8009 mL 1.6018 mL 4.0045 mL
40 mM 0.1201 mL 0.6007 mL 1.2013 mL 3.0034 mL
50 mM 0.0961 mL 0.4805 mL 0.9611 mL 2.4027 mL
60 mM 0.0801 mL 0.4004 mL 0.8009 mL 2.0022 mL
80 mM 0.0601 mL 0.3003 mL 0.6007 mL 1.5017 mL
100 mM 0.0481 mL 0.2403 mL 0.4805 mL 1.2013 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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Propargylcholine bromide Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Propargylcholine111755-76-1OthersNIH 3T3 cellsAlexa568propargylphosphatidylcholinesphingomyelinphospholipidsInhibitorinhibitorinhibit

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