2. Anti-infection Apoptosis
  3. Antibiotic Fungal Apoptosis
  4. Pyoluteorin

Pyoluteorin is an antibiotic that inhibits Oomycete fungi, including the plant pathogen Pythium ultimum, and suppresses plant diseases caused by this fungus. Pyoluteorin induces human triple-negative breast cancer MDA-MB-231 cells apoptosis in vitro. Pyoluteorin can be used for the research of human triple-negative breast cancer.

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Pyoluteorin Chemical Structure

Pyoluteorin Chemical Structure

CAS No. : 25683-07-2

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Based on 1 publication(s) in Google Scholar

Pyoluteorin Related Antibodies

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Description

Pyoluteorin is an antibiotic that inhibits Oomycete fungi, including the plant pathogen Pythium ultimum, and suppresses plant diseases caused by this fungus[1]. Pyoluteorin induces human triple-negative breast cancer MDA-MB-231 cells apoptosis in vitro. Pyoluteorin can be used for the research of human triple-negative breast cancer[2].

Cellular Effect
Cell Line Type Value Description References
MDCK IC50
4.8 μM
Compound: Pyoluteorin
Cytotoxicity against MDCK cells assessed as reduction in cell viability after 72 hrs by MTT assay
Cytotoxicity against MDCK cells assessed as reduction in cell viability after 72 hrs by MTT assay
[PMID: 30793902]
In Vitro

Pyoluteorin is an antifungal compound composed of a bichlorinated pyrrole linked to a resorcinol moiety, were identified within a 24-kb genomic region of Pseudomonas fluorescens Pf-5[1].
Pyoluteorin has significant cytotoxicity towards MCF-7 (IC50=1.84 μM). Pyoluteorin also displays significantly selective cytotoxicity against BT474, HCC1954, MAD-MB-468, MDA-MB-231, and MCF-10A cells with IC50s of 9.75±0.16, 0.94±0.01, 3.89±0.08, 0.97±0.01, and 57.01±0.76 μM, respectively[2].
Pyoluteorin (0.1-10 μM; for 24 hours) induces change of apoptosis-related protein expressions. Pyoluteorin-induced cell apoptosis in MDA-MB-231 is related to Bcl-2 family proteins and caspase cascade[2].
Pyoluteorin (0.1-10 μM; for 24 h) induces cell cycle arrest and apoptosis in human triple-negative breast cancer cells MDA-MB-231[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Pyoluteorin Related Antibodies

Cell Proliferation Assay[2]

Cell Line: Human triple-negative breast cancer cell MDA-MB-231
Concentration: 0, 0.032, 0.16, 0.8, 4, 20, 100 μM
Incubation Time: 24, 48, 72 hours
Result: Inhibited cells proliferation in a dose- and time-dependent manner.

Western Blot Analysis[2]

Cell Line: MDA-MB-231 cells
Concentration: 0.1, 0.3, 1, 3, 10 μM
Incubation Time: 24 hours
Result: The levels of the anti-apoptotic proteins Bcl-2, Bcl-XL and PARP were obviously decreased while the pro-apoptotic proteins BAX and caspase 3 were increased in a dose-dependent manner.
Molecular Weight

272.08

Formula

C11H7Cl2NO3
CAS No.
Appearance

Solid

Color

Off-white to yellow

SMILES

O=C(C1=C(C=CC=C1O)O)C2=CC(Cl)=C(N2)Cl
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, stored under nitrogen

*In solvent : -80°C, 6 months; -20°C, 1 month (stored under nitrogen)

Solvent & Solubility
In Vitro: 

DMSO : 125 mg/mL (459.42 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 3.6754 mL 18.3769 mL 36.7539 mL
5 mM 0.7351 mL 3.6754 mL 7.3508 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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Purity & Documentation

Purity: 99.90%

SDS (479 KB)

COA (254 KB) HNMR (134 KB) LCMS (93 KB)

Handling Instructions (2659 KB)
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 3.6754 mL 18.3769 mL 36.7539 mL 91.8847 mL
5 mM 0.7351 mL 3.6754 mL 7.3508 mL 18.3769 mL
10 mM 0.3675 mL 1.8377 mL 3.6754 mL 9.1885 mL
15 mM 0.2450 mL 1.2251 mL 2.4503 mL 6.1256 mL
20 mM 0.1838 mL 0.9188 mL 1.8377 mL 4.5942 mL
25 mM 0.1470 mL 0.7351 mL 1.4702 mL 3.6754 mL
30 mM 0.1225 mL 0.6126 mL 1.2251 mL 3.0628 mL
40 mM 0.0919 mL 0.4594 mL 0.9188 mL 2.2971 mL
50 mM 0.0735 mL 0.3675 mL 0.7351 mL 1.8377 mL
60 mM 0.0613 mL 0.3063 mL 0.6126 mL 1.5314 mL
80 mM 0.0459 mL 0.2297 mL 0.4594 mL 1.1486 mL
100 mM 0.0368 mL 0.1838 mL 0.3675 mL 0.9188 mL
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Pyoluteorin Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Pyoluteorin25683-07-2AntibioticFungalApoptosisOomycetefungiPythiumultimumfungusMDA-MB-231triple-negativebreastcancerapoptosiscellcyclearrestInhibitorinhibitorinhibit

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