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  4. Pyronin B

Pyronin B is an organic cationic dye used for the staining of bacteria, mycobacteria and ribonucleic acids. Pyronin B is also used as a small hydrophobic (SH) protein channel inhibitor.

For research use only. We do not sell to patients.

Pyronin B Chemical Structure

Pyronin B Chemical Structure

CAS No. : 2150-48-3

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Based on 1 publication(s) in Google Scholar

Pyronin B Related Antibodies

Top Publications Citing Use of Products

  • Biological Activity

  • Purity & Documentation

  • References

  • Customer Review

Description

Pyronin B is an organic cationic dye used for the staining of bacteria, mycobacteria and ribonucleic acids. Pyronin B is also used as a small hydrophobic (SH) protein channel inhibitor[1][2].

Cellular Effect
Cell Line Type Value Description References
HeLa IC50
2 μM
Compound: 2
Cytotoxicity against human HeLa cells assessed as reduction in cell viability incubated for 48 hrs by flow cytometry
Cytotoxicity against human HeLa cells assessed as reduction in cell viability incubated for 48 hrs by flow cytometry
[PMID: 35647549]
In Vitro

Pyronin B (0-15 μM) inhibits channel activity of approximately 60% of small hydrophobic (SH) proteins at a concentration of 10 μM, and the Kd value of 6.8 μM[1].
Pyronin B (0-0.25 μM) affects RSV replication in Vero cells in a dose-dependent manner, and its TCID50 (50% tissue culture infection dose) = 0 at the concentration of 0.25 μM[1].
Pyronin B (10 μM) can react with bromate in an oxidation reaction and the reaction rate increases dramatically with the addition of trace amounts of Ti (IV) with the characteristic absorption spectra of 555 nm[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Pyronin B Related Antibodies
Molecular Weight

358.90

Formula

C21H27ClN2O
CAS No.
Appearance

Solid

Color

Brown to black

SMILES

CCN(C1=CC2=[O+]C3=C(C=CC(N(CC)CC)=C3)C=C2C=C1)CC.[Cl-]
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, protect from light, stored under nitrogen

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)

Solvent & Solubility
In Vitro: 

DMSO : 20.83 mg/mL (58.04 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.7863 mL 13.9315 mL 27.8629 mL
5 mM 0.5573 mL 2.7863 mL 5.5726 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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In Vivo Dissolution Calculator
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Purity & Documentation

SDS (252 KB)

COA (247 KB)

Handling Instructions (2659 KB)
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 2.7863 mL 13.9315 mL 27.8629 mL 69.6573 mL
5 mM 0.5573 mL 2.7863 mL 5.5726 mL 13.9315 mL
10 mM 0.2786 mL 1.3931 mL 2.7863 mL 6.9657 mL
15 mM 0.1858 mL 0.9288 mL 1.8575 mL 4.6438 mL
20 mM 0.1393 mL 0.6966 mL 1.3931 mL 3.4829 mL
25 mM 0.1115 mL 0.5573 mL 1.1145 mL 2.7863 mL
30 mM 0.0929 mL 0.4644 mL 0.9288 mL 2.3219 mL
40 mM 0.0697 mL 0.3483 mL 0.6966 mL 1.7414 mL
50 mM 0.0557 mL 0.2786 mL 0.5573 mL 1.3931 mL
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Pyronin B Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Pyronin B2150-48-3DNA Staincationic dyenucleic acidsmall hydrophobic (SH) proteinchannel inhibitorInhibitorinhibitorinhibit

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