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  4. Pyrromethene 556

Pyrromethene 556  (Synonyms: PM556)

Cat. No.: HY-W248118 Purity: 99.03%
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Handling Instructions Technical Support

Pyrromethene 556 (PM556) is a green-fluorescent polar tracer dye. It is used for investigations of membrane fusion, lysis, and gap-junctional communication and to detect volume changes in cells or liposomes.

For research use only. We do not sell to patients.

Pyrromethene 556 Chemical Structure

Pyrromethene 556 Chemical Structure

CAS No. : 121461-69-6

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Description

Pyrromethene 556 (PM556) is a green-fluorescent polar tracer dye. It is used for investigations of membrane fusion, lysis, and gap-junctional communication and to detect volume changes in cells or liposomes.

Molecular Weight

466.20

Formula

C14H15BF2N2Na2O6S2
CAS No.
Appearance

Solid

Color

Yellow to orange

SMILES

O=S(C1=C(C)C2=C(C)C3=C(C)C(S(=O)(O[Na])=O)=C(C)[N-]3[B+3]([F-])([N]2=C1C)[F-])(O[Na])=O
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, sealed storage, away from moisture and light

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

Solvent & Solubility
In Vitro: 

DMSO : 41.67 mg/mL (89.38 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.1450 mL 10.7250 mL 21.4500 mL
5 mM 0.4290 mL 2.1450 mL 4.2900 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
Purity & Documentation

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 2.1450 mL 10.7250 mL 21.4500 mL 53.6251 mL
5 mM 0.4290 mL 2.1450 mL 4.2900 mL 10.7250 mL
10 mM 0.2145 mL 1.0725 mL 2.1450 mL 5.3625 mL
15 mM 0.1430 mL 0.7150 mL 1.4300 mL 3.5750 mL
20 mM 0.1073 mL 0.5363 mL 1.0725 mL 2.6813 mL
25 mM 0.0858 mL 0.4290 mL 0.8580 mL 2.1450 mL
30 mM 0.0715 mL 0.3575 mL 0.7150 mL 1.7875 mL
40 mM 0.0536 mL 0.2681 mL 0.5363 mL 1.3406 mL
50 mM 0.0429 mL 0.2145 mL 0.4290 mL 1.0725 mL
60 mM 0.0358 mL 0.1788 mL 0.3575 mL 0.8938 mL
80 mM 0.0268 mL 0.1341 mL 0.2681 mL 0.6703 mL
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Pyrromethene 556 Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Pyrromethene 556121461-69-6PM556Pyrromethene556Pyrromethene-556PM 556PM-556Fluorescent DyeInhibitorinhibitorinhibit

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