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RapiFluor-MS can be used a marker for LC-MS/MS analysis of N-glycans, which provides the highest MS signal enhancement for neutral glycans.

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RapiFluor-MS Chemical Structure

RapiFluor-MS Chemical Structure

CAS No. : 1429047-69-7

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Description

RapiFluor-MS can be used a marker for LC-MS/MS analysis of N-glycans, which provides the highest MS signal enhancement for neutral glycans[1][2][3][4].

In Vitro

Sample preparation:[4]
(1) Denaturation of glycoprotein;
(2) Deglycosylation using PNGase F;
(3) N-glycans labeling with RapiFluor-MS reagent for enhanced fluorescence and MS signal;
Dissolve 9 mg of RapiFluor-MS reagent in 110 μL of anhydrous DMF. Aspirate and dispense the solution or vortex to mix. Add 10 μL of RapiFluor-MS solution to the deglycosylated mixture in the 1 mL tube. Aspirate and dispense to mix.
(4) Purification using a SPE μElution plate in HILIC mode.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

427.45

Formula

C21H25N5O5

CAS No.
Appearance

Solid

Color

Light yellow to light brown

SMILES

O=C(ON1C(CCC1=O)=O)NC2=CC=C3N=C(C(NCCN(CC)CC)=O)C=CC3=C2

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

-20°C, sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Solvent & Solubility
In Vitro: 

DMSO : 12.5 mg/mL (29.24 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.3395 mL 11.6973 mL 23.3945 mL
5 mM 0.4679 mL 2.3395 mL 4.6789 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Purity & Documentation

Purity: ≥90.0%

References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 2.3395 mL 11.6973 mL 23.3945 mL 58.4864 mL
5 mM 0.4679 mL 2.3395 mL 4.6789 mL 11.6973 mL
10 mM 0.2339 mL 1.1697 mL 2.3395 mL 5.8486 mL
15 mM 0.1560 mL 0.7798 mL 1.5596 mL 3.8991 mL
20 mM 0.1170 mL 0.5849 mL 1.1697 mL 2.9243 mL
25 mM 0.0936 mL 0.4679 mL 0.9358 mL 2.3395 mL
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Product Name:
RapiFluor-MS
Cat. No.:
HY-138655
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