Acetylcholinesterase/ACHE Protein, Mouse (HEK293, His)

Acetylcholinesterase (ACHE) proteins exhibit multiple functions, including acetylcholinesterase activity and protein self-association. It regulates acetylcholine processing, receptor signaling and recycling. Acetylcholinesterase/ACHE Protein, Mouse (HEK293, His) is the recombinant mouse-derived Acetylcholinesterase/ACHE protein, expressed by HEK293 , with C-10*His labeled tag.

Acetylcholinesterase (ACHE) protein, with its diverse functional repertoire, exhibits acetylcholinesterase activity, identical protein binding activity, and the ability for protein self-association. Its involvement in positive regulation of cold-induced thermogenesis positions it upstream of critical processes such as acetylcholine catabolic processes, acetylcholine receptor signaling pathways, and the regulation of receptor recycling. ACHE is found in various cellular components, including the basement membrane, cell surface, and neuromuscular junction. Its expression spans across multiple structures, including the alimentary system, genitourinary system, musculature, nervous system, and sensory organs. Notably, human orthologs of ACHE are implicated in significant health conditions such as Alzheimer's disease, epilepsy, and myasthenia gravis, highlighting the importance of this protein in various physiological contexts. Broad expression patterns further emphasize its fundamental role in multiple tissues, particularly in the cerebellum and thymus.

Measured by its ability to cleave Acetylthiocholine. The specific activity is > 250 nmoL/min/μg, as measured under the described conditions.

Mouse

HEK293

C-10*His

P21836-1/NP_033729.1 (E32-L614)

11423  [NCBI]

EGREDPQLLVRVRGGQLRGIRLKAPGGPVSAFLGIPFAEPPVGSRRFMPPEPKRPWSGVLDATTFQNVCYQYVDTLYPGFEGTEMWNPNRELSEDCLYLNVWTPYPRPASPTPVLIWIYGGGFYSGAASLDVYDGRFLAQVEGAVLVSMNYRVGTFGFLALPGSREAPGNVGLLDQRLALQWVQENIAAFGGDPMSVTLFGESAGAASVGMHILSLPSRSLFHRAVLQSGTPNGPWATVSAGEARRRATLLARLVGCPPGGAGGNDTELIACLRTRPAQDLVDHEWHVLPQESIFRFSFVPVVDGDFLSDTPEALINTGDFQDLQVLVGVVKDEGSYFLVYGVPGFSKDNESLISRAQFLAGVRIGVPQASDLAAEAVVLHYTDWLHPEDPTHLRDAMSAVVGDHNVVCPVAQLAGRLAAQGARVYAYIFEHRASTLTWPLWMGVPHGYEIEFIFGLPLDPSLNYTTEERIFAQRLMKYWTNFARTGDPNDPRDSKSPQWPPYTTAAQQYVSLNLKPLEVRRGLRAQTCAFWNRFLPKLLSATDTLDEAERQWKAEFHRWSSYMVHWKNQFDHYSKQERCSDL

Approximately 80 kDa

• 
  Greater than 95% as determined by reducing SDS-PAGE.

Lyophilized powder.

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.