ACP5 Protein, Mouse (HEK293, His)

The ACP5 protein may have a crucial role in bone resorption, particularly in the enzymatic activities involved in breaking down bone tissue. ACP5 is linked to the osteoclastic trap, which has higher affinity for nucleotide tri- and diphosphates. Understanding ACP5's mechanisms in bone resorption and its interactions with nucleotide substrates could offer insights into its role in maintaining bone health. ACP5 Protein, Mouse (HEK293, His) is the recombinant mouse-derived ACP5 protein, expressed by HEK293 , with C-His labeled tag. The total length of ACP5 Protein, Mouse (HEK293, His) is 305 a.a., with molecular weight of ~35.8-38 kDa.

The ACP5 protein may play a crucial role in the process of bone resorption, indicating its involvement in the intricate mechanisms underlying the breakdown of bone tissue. Specifically, ACP5 is associated with the osteoclastic trap, which exhibits higher affinity for nucleotide tri- and diphosphates compared to other substrates. This suggests that ACP5 is intricately linked to the enzymatic activities that facilitate the degradation of bone matrix, potentially influencing the regulation of bone remodeling. Further exploration into the specific mechanisms by which ACP5 functions in bone resorption and its interactions with nucleotide substrates could provide valuable insights into its role in maintaining bone homeostasis and the potential implications for skeletal health.

Measured by its ability to cleave a substrate, p-Nitrophenyl phosphate (pNPP). The specific activity is 78579.56 pmol/min/μg.

Mouse

HEK293

C-His

Q05117 (A23-P327)

11433  [NCBI]

APTPTLRFVAVGDWGGVPNAPFHTAREMANAKEIARTVQTMGADFIMSLGDNFYFTGVHDASDKRFQETFEDVFSDRALRNIPWYVLAGNHDHLGNVSAQIAYSKISKRWNFPSPYYRLRFKIPRTNITVAIFMLDTVMLCGNSDDFASQQPKMPRDLGVARTQLSWLKKQLAAAKEDYVLVAGHYPIWSIAEHGPTRCLVKNLRPLLATYGVTAYLCGHDHNLQYLQDENGVGYVLSGAGNFMDPSVRHQRKVPNGYLRFHYGSEDSLGGFTHVEISPKEMTIIYVEASGKSLFKTSLPRRPRP

Approximately 35.8-38 kDa

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  Greater than 95% as determined by reducing SDS-PAGE.

Lyophilized powder

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.