1. Recombinant Proteins
  2. Enzymes & Regulators
  3. Hydrolases (EC 3) ADAMs/ADAMTSs
  4. ADAM17/TACE
  5. ADAM17/TACE Protein, Mouse (HEK293, His)

ADAM17/TACE Protein, Mouse (HEK293, His)

Cat. No.: HY-P78717
SDS COA Handling Instructions

The ADAM17/TACE protein cleaves TNF-α, releasing it from the membrane and activating various cell surface proteins such as JAM3, ACE2, and the p75 TNF receptor. It also triggers the Notch pathway by cleaving Notch into membrane-associated fragments. ADAM17/TACE Protein, Mouse (HEK293, His) is the recombinant mouse-derived ADAM17/TACE protein, expressed by HEK293 , with C-His labeled tag. The total length of ADAM17/TACE Protein, Mouse (HEK293, His) is 546 a.a..

For research use only. We do not sell to patients.

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  • Biological Activity

  • Technical Parameters

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Description

The ADAM17/TACE protein cleaves TNF-α, releasing it from the membrane and activating various cell surface proteins such as JAM3, ACE2, and the p75 TNF receptor. It also triggers the Notch pathway by cleaving Notch into membrane-associated fragments. ADAM17/TACE Protein, Mouse (HEK293, His) is the recombinant mouse-derived ADAM17/TACE protein, expressed by HEK293 , with C-His labeled tag. The total length of ADAM17/TACE Protein, Mouse (HEK293, His) is 546 a.a..

Background

ADAM17/TACE Protein is responsible for cleaving the membrane-bound precursor of TNF-alpha, converting it into its mature soluble form. It also plays a crucial role in the proteolytic release of various cell-surface proteins, including JAM3, ACE2, p75 TNF-receptor, interleukin 1 receptor type II, p55 TNF-receptor, transforming growth factor-alpha, L-selectin, growth hormone receptor, MUC1, and the amyloid precursor protein. Additionally, ADAM17/TACE activates the Notch pathway by mediating the cleavage of Notch, generating a membrane-associated intermediate fragment known as notch extracellular truncation (NEXT). In hemostasis, it contributes by shedding the platelet glycoprotein Ib alpha chain (GP1BA), while also facilitating the proteolytic cleavage and release of secreted forms of LAG3 and IL6R. Furthermore, ADAM17/TACE mediates the proteolytic cleavage and shedding of FCGR3A upon stimulation of natural killer cells, allowing for enhanced NK cell motility and detachment from opsonized target cells.

Biological Activity

Measured by its ability to cleave 10μM fluorogenic peptide substrate Mca-PLAQAV-Dpa-RSSSR-NH2 that incubate at room temperature in kinetic mode for 5 minutes. The specific activity is 1818.15 pmol/min/µg.

Species

Mouse

Source

HEK293

Tag

C-6*His

Accession

Q9Z0F8 (P18-D563)

Gene ID
Molecular Construction
N-term
ADAM17 (P18-D563)
Accession # Q9Z0F8
6*His
C-term
Synonyms
ADAM17; TACE; CD156b
AA Sequence

RPPEEAGSGSHPRLEKLDSLLSDYDILSLANIQQHSIRKRDLQSATHLETLLTFSALKRHFKLYLTSSTERFSQNLRVVVVDGKEESEYSVKWQNFFSGHVVGEPDSRVLAHIGDDDVTVRINTDGAEYNVEPLWRFVNDTKDKRMLVYKSEDIKDFSRLQSPKVCGYLNADSEELLPKGLIDREPSEEFVRRVKRPRPPEEAGSGSHPRLEKLDSLLSDYDILSLANIQQHSIRKRDLQSATHLETLLTFSALKRHFKLYLTSSTERFSQNLRVVVVDGKEESEYSVKWQNFFSGHVVGEPDSRVLAHIGDDDVTVRINTDGAEYNVEPLWRFVNDTKDKRMLVYKSEDIKDFSRLQSPKVCGYLNADSEELLPKGLIDREPSEEFVRRVKRRAEPNPLKNTCKLLVVADHRFYKYMGRGEESTTTNYLIELIDRVDDIYRNTSWDNAGFKGYGVQIEQIRILKSPQEVKPGERHFNMAKSFPNEEKDAWDVKMLLEQFSFDIAEEASKVCLAHLFTYQDFDMGTLGLAYVGSPRANSHGGVCPKAYYNPTVKKNIYLNSGLTSTKNYGKTILTKEADLVTTHELGHNFGAEHDPDGLAECAPNEDQGGKYVMYPIAVSGDHENNKMFSNCSKQSIYKTIESKAQECFQERSNKVCGNSRVDEGEECDPGIMYLNNDTCCNSDCTLKPGVQCSDRNSPCCKNCQFETAQKKCQEAINATCKGVSYCTGNSSECPPPGDAED

Molecular Weight

Approximately 55-75 & 25 kDa due to the glycosylation.

Purity

Greater than 95% as determined by reducing SDS-PAGE.

Appearance

Lyophilized powder.

Formulation

Lyophilized a 0.22 μm filtered solution of PBS, pH 7.4, 8% trehalose.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Storage & Stability

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Documentation

ADAM17/TACE Protein, Mouse (HEK293, His) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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ADAM17/TACE Protein, Mouse (HEK293, His)
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