AE-binding protein 1/Aebp1 Protein, Mouse (His-SUMO)

AE-binding protein 1/Aebp1 Protein, Mouse (His-SUMO) plays a critical role in regulating adipogenesis, and the proinflammation process in macrophages, including macrophage cholesterol homeostasis, foam cell formation and the development of atherosclerosis^[1][2].

AEBP1 acts as a transcriptional repressor that impedes macrophage cholesterol efflux, promoting foam cell formation, via PPARgamma1 and LXRalpha down-regulation^[1].
Silencing AEBP1 markedly suppresses the proliferation, migration, invasion, metastasis and epithelial-mesenchymal transition of GC cells. Moreover, we demonstrats that knockdown of AEBP1 in GC cells leads to inhibition of the NF-κB pathway by hampering the degradation of IκBα^[2].

Mouse

E. coli

N-His;N-SUMO

Q640N1 (26Q-370P)

11568  [NCBI]

QTVLTDDEIEEFLEGFLSELETQSPPREDDVEVQPLPEPTQRPRKSKAGGKQRADVEVPPEKNKDKEKKGKKDKGPKATKPLEGSTRPTKKPKEKPPKATKKPKEKPPKATKKPKEKPPKATKKPKEKPPKATKRPSAGKKFSTVAPLETLDRLLPSPSNPSAQELPQKRDTPFPNAWQGQGEETQVEAKQPRPEPEEETEMPTLDYNDQIEKEDYEDFEYIRRQKQPRPTPSRRRLWPERPEEKTEEPEERKEVEPPLKPLLPPDYGDSYVIPNYDDLDYYFPHPPPQKPDVGQEVDEEKEEMKKPKKEGSSPKEDTEDKWTVEKNKDHKGPRKGEELEEEWAP

Approximately 70 kDa.The reducing (R) protein migrat es as 70 kDa in SDS-PAGE may be due to relative charge.

Greater than 90% as determined by reducing SDS-PAGE.

Lyophilized powder.

Lyophilized after extensive dialysis against solution in 20 mM Tris-HCl, 0.5 M NaCl, 6% Trehalose, pH 8.0.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.

• [1]. Amin Majdalawieh, et al. Adipocyte enhancer-binding protein 1 is a potential novel atherogenic factor involved in macrophage cholesterol homeostasis and inflammation. Proc Natl Acad Sci U S A. 2006 Feb 14;103(7):2346-51.  [Content Brief]

  [2]. Jun-Yan Liu, et al. AEBP1 promotes epithelial-mesenchymal transition of gastric cancer cells by activating the NF-κB pathway and predicts poor outcome of the patients. Sci Rep. 2018 Aug 10;8(1):11955.  [Content Brief]