1. Recombinant Proteins
  2. CD Antigens Receptor Proteins Biotinylated Proteins
  3. Macrophage CD Proteins Stem Cell CD Proteins Epithelial cell CD Proteins Cell Adhesion Molecules (CAMs)
  4. ALCAM/CD166 Immunoglobulin-like Cell Adhesion Molecules
  5. Activated Leukocyte Cell Adhesion Molecule (ALCAM)
  6. ALCAM/CD166 Protein, Human (Biotinylated, HEK293, His-Avi)

ALCAM/CD166 Protein, Human (Biotinylated, HEK293, His-Avi)

Cat. No.: HY-P78057
Handling Instructions

The ALCAM/CD166 protein is a cell adhesion molecule that mediates heterotypic and homotypic cell-to-cell contacts, promoting T cell activation, immune synapse formation, and LILRB4-mediated inhibition of T cell proliferation. ALCAM/CD166 Protein, Human (Biotinylated, HEK293, His-Avi) is the recombinant human-derived ALCAM/CD166 protein, expressed by HEK293 , with C-Avi, C-His labeled tag. The total length of ALCAM/CD166 Protein, Human (Biotinylated, HEK293, His-Avi) is 499 a.a., with molecular weight of 72-80 kDa.

For research use only. We do not sell to patients.

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Description

The ALCAM/CD166 protein is a cell adhesion molecule that mediates heterotypic and homotypic cell-to-cell contacts, promoting T cell activation, immune synapse formation, and LILRB4-mediated inhibition of T cell proliferation. ALCAM/CD166 Protein, Human (Biotinylated, HEK293, His-Avi) is the recombinant human-derived ALCAM/CD166 protein, expressed by HEK293 , with C-Avi, C-His labeled tag. The total length of ALCAM/CD166 Protein, Human (Biotinylated, HEK293, His-Avi) is 499 a.a., with molecular weight of 72-80 kDa.

Background

ALCAM/CD166 protein serves as a pivotal cell adhesion molecule, orchestrating a spectrum of cellular interactions. It mediates both heterotypic cell-cell contacts through its interaction with CD6 and homotypic cell-cell contacts, promoting T-cell activation and proliferation. ALCAM contributes significantly to the formation and maturation of the immunological synapse by engaging with CD6. Additionally, it facilitates homotypic interactions with cells expressing ALCAM. Acting as a ligand for the LILRB4 receptor, ALCAM enhances LILRB4-mediated inhibition of T cell proliferation. The protein is indispensable for normal hematopoietic stem cell engraftment in the bone marrow and mediates the attachment of dendritic cells onto endothelial cells through homotypic interactions. ALCAM plays a vital role in inhibiting endothelial cell migration while promoting endothelial tube formation. It is crucial for the organization of the lymph vessel network and hematopoiesis, ensuring normal numbers of hematopoietic stem cells in the bone marrow. ALCAM's diverse functions extend to promoting in vitro osteoblast proliferation and differentiation, as well as influencing neurite extension, axon growth, and axon guidance. Notably, it inhibits membrane-bound isoforms by competing for the same interaction partners, restricts cell attachment via homotypic interactions, and simultaneously promotes endothelial cell migration while inhibiting endothelial cell tube formation.

Species

Human

Source

HEK293

Tag

C-Avi;C-His

Accession

Q13740 (W28-A526)

Gene ID

214  [NCBI]

Molecular Construction
N-term
ALCAM (W28-A526)
Accession # Q13740
His-Avi
C-term
Synonyms
CD166; ALCAM; MEMD; CD6L; KG-CAM
Molecular Weight

72-80 kDa

Purity

Greater than 95% as determined by Tris-Bis PAGE.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Documentation
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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Product Name:
ALCAM/CD166 Protein, Human (Biotinylated, HEK293, His-Avi)
Cat. No.:
HY-P78057
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