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  5. ALDH3A1 Protein, Human (HEK293, His)

ALDH3A1 Protein, Human (HEK293, His)

Cat. No.: HY-P7477
Handling Instructions

ALDH3A1 Protein, Human (HEK293, His) is a human recombinant ALDH3A1 with a His tag at the N-terminus. ALDH3A1 Protein, Human (HEK293, His) is expressed in HEK293 cells.

For research use only. We do not sell to patients.

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Description

ALDH3A1 Protein, Human (HEK293, His) is a human recombinant ALDH3A1 with a His tag at the N-terminus. ALDH3A1 Protein, Human (HEK293, His) is expressed in HEK293 cells.
Background

Aldehyde dehydrogenase 3A1 (ALDH3A1) is an important member of the ALDH superfamily, which consists of NAD(P)+- dependent enzymes that oxidize a wide variety of endogenously produced and exogenously derived aldehydes to their corresponding carboxylic acids. ALDH3A1 is often overexpressed in esophagus-mucosa, minor salivary gland, nasal epithelium, tonsil and oral epithelium. ALDH3A1 has been shown to be upregulated in several cancer types, such as stomach cancer, lung cancer or oral squamous cell carcinoma (OSCC)[1].
Species

Human
Source

HEK293
Tag

C-6*His
Accession

P30838 (M1-Q452)
Gene ID

218  [NCBI]
Molecular Construction
N-term
ALDH3A1 (M1-Q452)
Accession # P30838
6*His
C-term
Synonyms
rHuAldehyde dehydrogenase family 3 member A1, His; ALDH-3A1; Aldehyde Dehydrogenase 3 member A1
AA Sequence

MSKISEAVKRARAAFSSGRTRPLQFRIQQLEALQRLIQEQEQELVGALAADLHKNEWNAYYEEVVYVLEEIEYMIQKLPEWAADEPVEKTPQTQQDELYIHSEPLGVVLVIGTWNYPFNLTIQPMVGAIAAGNSVVLKPSELSENMASLLATIIPQYLDKDLYPVINGGVPETTELLKERFDHILYTGSTGVGKIIMTAAAKHLTPVTLELGGKSPCYVDKNCDLDVACRRIAWGKFMNSGQTCVAPDYILCDPSIQNQIVEKLKKSLKEFYGEDAKKSRDYGRIISARHFQRVMGLIEGQKVAYGGTGDAATRYIAPTILTDVDPQSPVMQEEIFGPVLPIVCVRSLEEAIQFINQREKPLALYMFSSNDKVIKKMIAETSSGGVAANDVIVHITLHSLPFGGVGNSGMGSYHGKKSFETFSHRRSCLVRPLMNDEGLKVRYPPSPAKMTQHHHHHHH

Molecular Weight

Approximately 58.0 kDa
Purity

Greater than 80% as determined by reducing SDS-PAGE.
Endotoxin Level

<1 EU/μg, determined by LAL method.
Documentation
Handling Instructions (2659 KB)
References

ALDH3A1 Protein, Human (HEK293, His) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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