2. Recombinant Proteins
  3. Enzymes & Regulators Biotinylated Proteins
  4. Hydrolases (EC 3) Phosphatase
  5. Alkaline Phosphatase
  6. Alkaline Phosphatase/ALPG Protein, Human (Biotinylated, HEK293, His-Avi)

Alkaline Phosphatase/ALPG Protein, Human (Biotinylated, HEK293, His-Avi)

Cat. No.: HY-P700951
Handling Instructions

The Alkaline Phosphatase/ALPG protein, an enzyme, hydrolyzes phosphate compounds, breaking down various molecules. It acts as an alkaline phosphatase, cleaving phosphate esters and rendering molecules inactive. This versatile enzyme is vital in processes like bone mineralization, cellular signaling, and metabolism. Alkaline Phosphatase/ALPG Protein, Human (Biotinylated, HEK293, His-Avi) is the recombinant human-derived Alkaline Phosphatase/ALPG protein, expressed by HEK293, with C-Avi, C-His labeled tag. The total length of Alkaline Phosphatase/ALPG Protein, Human (Biotinylated, HEK293, His-Avi) is 484 a.a., with molecular weight of 56-70 kDa.

For research use only. We do not sell to patients.

Surface Plasmon Resonance (SPR) Assay Service   Protein Expression Service

Size Stock
50 μg   Get quote  
100 μg   Get quote  

* Please select Quantity before adding items.

Top Publications Citing Use of Products

  • Biological Activity

  • Technical Parameters

  • Properties

  • Documentation

Description

The Alkaline Phosphatase/ALPG protein, an enzyme, hydrolyzes phosphate compounds, breaking down various molecules. It acts as an alkaline phosphatase, cleaving phosphate esters and rendering molecules inactive. This versatile enzyme is vital in processes like bone mineralization, cellular signaling, and metabolism. Alkaline Phosphatase/ALPG Protein, Human (Biotinylated, HEK293, His-Avi) is the recombinant human-derived Alkaline Phosphatase/ALPG protein, expressed by HEK293, with C-Avi, C-His labeled tag. The total length of Alkaline Phosphatase/ALPG Protein, Human (Biotinylated, HEK293, His-Avi) is 484 a.a., with molecular weight of 56-70 kDa.
Background

The Alkaline Phosphatase/ALPG protein is an enzyme that possesses the capability to hydrolyze a range of phosphate compounds. It exhibits the ability to break down various phosphate molecules through its enzymatic activity. This protein acts as an alkaline phosphatase, catalyzing the hydrolysis of phosphate esters in an alkaline environment. Its enzymatic activity allows it to cleave phosphate groups from molecules such as nucleotides, proteins, and phospholipids, rendering them inactive. This versatile enzyme plays a vital role in various physiological processes, including bone mineralization, cellular signaling, and metabolism.
Species

Human
Source

HEK293
Tag

C-Avi;C-His
Accession

P10696 (I20-D503)
Gene ID

251  [NCBI]
Molecular Construction
N-term
ALPG (I20-D503)
Accession # P10696
His-Avi
C-term
Synonyms
Alkaline Phosphatase; ALP-1; Alkaline phosphatase, placental-like; GCAP; PLAP-like; ALPG; ALPPL; ALPPL2
Molecular Weight

56-70 kDa
Purity

Greater than 95% as determined by reducing SDS-PAGE.
Endotoxin Level

<1 EU/μg, determined by LAL method.
Documentation
Handling Instructions (2659 KB)

Alkaline Phosphatase/ALPG Protein, Human (Biotinylated, HEK293, His-Avi) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

  • Specific Activity Calculator

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

Your Recently Viewed Products:

Inquiry Online

Your information is safe with us. * Required Fields.

Product Name

  

Salutation

Applicant Name *

 

Email Address *

Phone Number *

 

Organization Name *

Department *

 

Requested quantity *

Country or Region *

      

Remarks

Bulk Inquiry

Inquiry Information

Product Name:
Alkaline Phosphatase/ALPG Protein, Human (Biotinylated, HEK293, His-Avi)
Cat. No.:
HY-P700951
Quantity:
MCE Japan Authorized Agent:

Request for HNMR Report

Please fill out this form to request the QC report. We will send it to your Email address shortly.

Your information is safe with us. * Required Fields.

Product Name

  

Lot #

Applicant Name *

 

Country or Region *

Email Address *

     

Phone Number *

 

Organization Name *

Remarks

Request for HNMR Report

We have received your request and will respond to you as soon as possible.