Alkaline Phosphatase/ALPG Protein, Human (HEK293, His)

The Alkaline Phosphatase/ALPG protein, an enzyme, hydrolyzes phosphate compounds, breaking down various molecules. It acts as an alkaline phosphatase, cleaving phosphate esters and rendering molecules inactive. This versatile enzyme is vital in processes like bone mineralization, cellular signaling, and metabolism. Alkaline Phosphatase/ALPG Protein, Human (HEK293, His) is the recombinant human-derived Alkaline Phosphatase/ALPG protein, expressed by HEK293 , with C-His labeled tag. The total length of Alkaline Phosphatase/ALPG Protein, Human (HEK293, His) is 484 a.a., with molecular weight of 58-68 kDa.

The Alkaline Phosphatase/ALPG protein is an enzyme that possesses the capability to hydrolyze a range of phosphate compounds. It exhibits the ability to break down various phosphate molecules through its enzymatic activity. This protein acts as an alkaline phosphatase, catalyzing the hydrolysis of phosphate esters in an alkaline environment. Its enzymatic activity allows it to cleave phosphate groups from molecules such as nucleotides, proteins, and phospholipids, rendering them inactive. This versatile enzyme plays a vital role in various physiological processes, including bone mineralization, cellular signaling, and metabolism.

1.The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.
2.Immobilized Human Alkaline Phosphatase (Germ type) , His Tag at 0.5 μg/mL (100 μl/well) on the plate. Dose response curve for Anti-Alkaline Phosphatase Antibody, hFc Tag with the EC₅₀ of ≤8 ng/mL determined by ELISA.

Human

HEK293

C-His

P10696 (I20-D503)

251  [NCBI]

58-68 kDa

Greater than 95% as determined by reducing SDS-PAGE.

Lyophilized powder

Lyophilized from 0.22 μm filtered solution in 25 mM Tris, 100 mM NaCl, 1 mM MgCl₂ (pH 7.5). Normally 8% trehalose is added as protectant before lyophilization.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.