1. Recombinant Proteins
  2. Cytokines and Growth Factors Animal-free Recombinant Proteins
  3. Animal-Free HDGF Protein, Mouse (His)

Animal-Free HDGF Protein, Mouse (His)

Cat. No.: HY-P700181AF
COA Handling Instructions

HDGF Protein, a transcriptional repressor, promotes fibroblast mitogenic activity. Existing as a monomer or domain-swapped homodimer, it interacts with nuclear proteins NCL and YBX1/YB1. Animal-Free HDGF Protein, Mouse (His) is the recombinant mouse-derived animal-FreeHDGF protein, expressed by E. coli , with C-His labeled tag. The total length of Animal-Free HDGF Protein, Mouse (His) is 237 a.a., with molecular weight of ~27.08 kDa.

Animal-free recombinant proteins offer high consistency and stability, whithout using or contacting of any animal-derived materials and reagents.

For research use only. We do not sell to patients.

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Size Price Stock Quantity
10 μg $150 In-stock
50 μg $450 In-stock
100 μg   Get quote  

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Top Publications Citing Use of Products
  • Biological Activity

  • Technical Parameters

  • Properties

  • Documentation

Description

HDGF Protein, a transcriptional repressor, promotes fibroblast mitogenic activity. Existing as a monomer or domain-swapped homodimer, it interacts with nuclear proteins NCL and YBX1/YB1. Animal-Free HDGF Protein, Mouse (His) is the recombinant mouse-derived animal-FreeHDGF protein, expressed by E. coli , with C-His labeled tag. The total length of Animal-Free HDGF Protein, Mouse (His) is 237 a.a., with molecular weight of ~27.08 kDa.

Background

HDGF Protein functions as a transcriptional repressor and exhibits mitogenic activity for fibroblasts. This heparin-binding protein can exist as a monomer and as a domain-swapped homodimer. Additionally, it interacts with nuclear proteins NCL and YBX1/YB1.

Species

Mouse

Source

E. coli

Tag

C-His

Accession

P51859 (M1-L237)

Gene ID
Molecular Construction
N-term
HDGF (M1-L237)
Accession # P51859
His
C-term
Synonyms
Hepatoma-Derived Growth Factor; HDGF; High Mobility Group Protein 1-Kike 2; HMG-1L2; HDGF; HMG1L2
AA Sequence

MSRSNRQKEYKCGDLVFAKMKGYPHWPARIDEMPEAAVKSTANKYQVFFFGTHETAFLGPKDLFPYEESKEKFGKPNKRKGFSEGLWEIENNPTVKASGYQSSQKKSCAAEPEVEPEAHEGDGDKKGSAEGSSDEEGKLVIDEPAKEKNEKGTLKRRAGDVLEDSPKRPKESGDHEEEDKEIAALEGERPLPVEVEKNSTPSEPDSGQGPPAEEEEGEEEAAKEEAEAQGVRDHESL

Molecular Weight

Approximately 27.08 kDa

Purity

Greater than 95% as determined by reducing SDS-PAGE.

Appearance

Lyophilized powder.

Formulation

Lyophilized from a solution containing 1X PBS, pH 7.4, trehalose.

Endotoxin Level

<0.1 EU per 1 μg of the protein by the LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.

Storage & Stability

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Documentation

Animal-Free HDGF Protein, Mouse (His) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

  • Specific Activity Calculator

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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Product Name:
Animal-Free HDGF Protein, Mouse (His)
Cat. No.:
HY-P700181AF
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