Animal-Free IL-26/AK155 Protein, Human (His)

The IL-26/AK155 monomeric protein may play a crucial role in mucosal immunity, and its proinflammatory functions are associated with inflammatory bowel disease. Functionally, it activates various signaling pathways, induces the expression of immune mediators, and reduces intestinal epithelial cell proliferation. Animal-Free IL-26/AK155 Protein, Human (His) is the recombinant human-derived animal-FreeIL-26/AK155 protein, expressed by E. coli , with C-His labeled tag. The total length of Animal-Free IL-26/AK155 Protein, Human (His) is 150 a.a., with molecular weight of ~18.53 kDa.

The IL-26/AK155 Monomer protein is implicated in potentially playing a crucial role in local mechanisms of mucosal immunity, suggesting its involvement in the intricate processes of immune regulation within mucosal tissues. It appears to have a pro-inflammatory function and may be associated with inflammatory bowel disease, underscoring its significance in inflammatory processes in the gastrointestinal tract. Functionally, IL-26/AK155 Monomer activates various signaling pathways, including STAT1 and STAT3, MAPK1/3 (ERK1/2), JUN, and AKT, suggesting a broad impact on cellular responses. It induces the expression of SOCS3, TNF-alpha, and IL-8, along with the secretion of IL-8 and IL-10, as well as the surface expression of ICAM1, highlighting its involvement in the regulation of immune and inflammatory mediators. Additionally, IL-26/AK155 Monomer demonstrates the ability to decrease the proliferation of intestinal epithelial cells. Notably, its activity is inhibited by heparin, adding a layer of regulatory control. The structural arrangement involves the formation of homodimers, emphasizing the importance of its oligomeric state in mediating cellular responses and signaling cascades within the context of mucosal immunity.

Measure by its ability to induce IL-10 secretion in COLO205 cells. The ED₅₀ for this effect is <150 ng/mL

Human

E. coli

C-His

Q9NPH9 (K22-Q171)

55801  [NCBI]

MKHKQSSFTKSCYPRGTLSQAVDALYIKAAWLKATIPEDRIKNIRLLKKKTKKQFMKNCQFQEQLLSFFMEDVFGQLQLQGCKKIRFVEDFHSLRQKLSHCISCASSAREMKSITRMKRIFYRIGNKGIYKAISELDILLSWIKKLLESSQ

Approximately 18.53 kDa

Greater than 95% as determined by reducing SDS-PAGE.

Lyophilized powder.

Lyophilized from a solution containing 1X PBS, pH 8.0, trehalose.

<0.1 EU per 1 μg of the protein by the LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US;may vary elsewhere.