Animal-Free IL-34 Protein, Human (His)

IL-34 protein is an important cytokine that promotes proliferation, survival, and differentiation of monocytes and macrophages. It plays a key role in innate immunity and inflammation by promoting the release of pro-inflammatory chemokines. Animal-Free IL-34 Protein, Human (His) is the recombinant human-derived animal-FreeIL-34 protein, expressed by E. coli , with C-His labeled tag.

The IL-34 Protein serves as a crucial cytokine, fostering the proliferation, survival, and differentiation of monocytes and macrophages. Its role extends to promoting the release of pro-inflammatory chemokines, thereby playing a pivotal part in innate immunity and inflammatory processes. Moreover, IL-34 assumes a critical role in the regulation of osteoclast proliferation and differentiation, impacting bone resorption. Through signaling via CSF1R and its downstream effectors, IL-34 stimulates the phosphorylation of MAPK1/ERK2 and MAPK3/ERK1. Existing as a homodimer, IL-34 interacts specifically with CSF1R, further contributing to its intricate regulatory network.

Measure by its ability to induce human peripheral blood monocytes (PBMCs) proliferation. The ED₅₀ for this effect is <30 pg/mL.

Human

E. coli

C-His

Q6ZMJ4-1 (N21-P242)

146433  [NCBI]

MNEPLEMWPLTQNEECTVTGFLRDKLQYRSRLQYMKHYFPINYKISVPYEGVFRIANVTRLQRAQVSERELRYLWVLVSLSATESVQDVLLEGHPSWKYLQEVETLLLNVQQGLTDVEVSPKVESVLSLLNAPGPNLKLVRPKALLDNCFRVMELLYCSCCKQSSVLNWQDCEVPSPQSCSPEPSLQYAATQLYPPPPWSPSSPPHSTGSVRPVRAQGEGLLP

Approximately 26.17 kDa under reducing (R) condition.

Greater than 95% as determined by reducing SDS-PAGE.

Lyophilized powder.

Lyophilized from a solution containing 1X PBS, pH 8.0, trehalose.

<0.1 EU per 1 μg of the protein by the LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.