Animal-Free LIX/CXCL5 Protein, Mouse (His)

The LIX/CXCL5 protein may recruit inflammatory cells to injured or infected tissues, suggesting a role in the immune response.Both GCP-2(1-78) and the more potent GCP-2(9-78) variant attract and activate neutrophils.Animal-Free LIX/CXCL5 Protein, Mouse (His) is the recombinant mouse-derived animal-FreeLIX/CXCL5 protein, expressed by E.coli , with N-His labeled tag.

The Animal-Free LIX/CXCL5 protein is implicated in potentially participating in the recruitment of inflammatory cells to injured or infected tissue, suggesting its role in the immune response. Both GCP-2(1-78) and the more potent GCP-2(9-78) variants of this protein play a role in attracting neutrophils and are involved in the activation of these immune cells. Structurally, the protein exists as a monomer and can also form homodimers, underscoring its versatility and potential for molecular interactions. The ability of Animal-Free LIX/CXCL5 to attract and activate neutrophils highlights its significance in orchestrating immune responses and cellular processes associated with inflammation.

Measure by its ability to chemoattract BaF3 cells transfected with human CXCR2. The ED₅₀ for this effect is <100 ng/mL

Mouse

E. coli

N-His

P50228 (V45-A118)

20311  [NCBI]

VIAATELRCVCLTVTPKINPKLIANLEVIPAGPQCPTVEVIAKLKNQKEVCLDPEAPVIKKIIQKILGSDKKKA

Approximately 8.92 kDa

Greater than 95% as determined by reducing SDS-PAGE.

Lyophilized powder.

Lyophilized from a solution containing 1X PBS, pH 7.4, trehalose.

<0.1 EU per 1 μg of the protein by the LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.