Apolipoprotein A-I/APOA1 Protein, Mouse (240a.a, HEK293, Fc)

Apolipoprotein A-I (APOA1) Protein, pivotal in cholesterol reverse transport, facilitates efflux from tissues and acts as a crucial cofactor for lecithin cholesterol acyltransferase (LCAT), promoting cholesterol excretion. As a homodimer, APOA1 is integral to the sperm activating protein complex (SPAP), interacting with APOA1BP, CLU, NDRG1, SCGB3A2, NAXE, and YJEFN3, demonstrating diverse molecular associations. Its involvement extends beyond cholesterol metabolism to influence cellular processes like spermatozoa motility and protein interactions. Apolipoprotein A-I/APOA1 Protein, Mouse (240a.a, HEK293, Fc) is the recombinant mouse-derived Apolipoprotein A-I/APOA1 protein, expressed by HEK293, with C-hFc labeled tag. The total length of Apolipoprotein A-I/APOA1 Protein, Mouse (240a.a, HEK293, Fc) is 240 a.a., with molecular weight of 55-65 kDa.

Apolipoprotein A-I (APOA1) Protein plays a pivotal role in the reverse transport of cholesterol, facilitating its efflux from tissues and functioning as a crucial cofactor for lecithin cholesterol acyltransferase (LCAT) to promote cholesterol excretion from tissues to the liver. This protein exists as a homodimer and is part of the sperm activating protein complex (SPAP), which includes APOA1, an immunoglobulin heavy chain, an immunoglobulin light chain, and albumin. APOA1 also interacts with APOA1BP and CLU, contributing to its diverse molecular associations. Additionally, it engages with NDRG1, SCGB3A2, NAXE, and YJEFN3, further highlighting its involvement in various cellular processes beyond cholesterol metabolism, including spermatozoa motility and protein complex interactions.

Mouse

HEK293

C-hFc

Q00623 (D25-Q264)

11806  [NCBI]

55-65 kDa

Greater than 95% as determined by Tris-Bis PAGE.

Lyophilized powder

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.