ARMET/MANF Protein, Mouse (HEK293, His)

ARMET/MANF proteins regulate the survival of dopaminergic neurons in the ventral midbrain and modulate GABAergic transmission to dopaminergic neurons in the substantia nigra. It enhances GABA inhibitory currents and inhibits cell proliferation while preventing ER stress-induced cell death. ARMET/MANF Protein, Mouse (HEK293, His) is the recombinant mouse-derived ARMET/MANF protein, expressed by HEK293 , with C-His labeled tag.

ARMET/MANF protein is a vital regulator of dopaminergic neuron survival in the ventral mid-brain, while also modulating GABAergic transmission to dopaminergic neurons in the substantia nigra. It enhances both spontaneous and evoked GABAergic inhibitory postsynaptic currents in these neurons. Additionally, it inhibits cell proliferation and protects against cell death induced by ER stress. In normal conditions, ARMET/MANF protein is retained in the ER/sarcoplasmic reticulum through its association with the chaperone protein HSPA5. However, it is upregulated and secreted by the ER/SR in response to ER stress and hypoxia. Upon secretion, it directly binds to 3-O-sulfogalactosylceramide, a lipid sulfatide on the outer cell membrane of target cells, facilitating its uptake through endocytosis. This sulfatide binding is crucial for ARMET/MANF's role in alleviating ER stress and cell toxicity under hypoxic and ER stress conditions. ARMET/MANF protein also interacts with HSPA5 and is a component of a complex that includes CRELD2, MATN3, and PDIA4.

Measured in a cell proliferation assay using rat C6 cells. The ED₅₀ for this effect is 1.457 μg/mL.

Mouse

HEK293

C-6*His

Q9CXI5/NP_083379.2 (L22-L179)

74840  [NCBI]

LRPGDCEVCISYLGRFYQDLKDRDVTFSPATIEEELIKFCREARGKENRLCYYIGATDDAATKIINEVSKPLAHHIPVEKICEKLKKKDSQICELKYDKQIDLSTVDLKKLRVKELKKILDDWGEMCKGCAEKSDYIRKINELMPKYAPKAASARTDL

Approximately 20 kDa

Greater than 95% as determined by reducing SDS-PAGE

Lyophilized powder.

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.