ATF1 Protein, Human (His)

ATF1 Protein, Human (His) expressed in E. coli with a His tag. ATF1 is a member of the ATF/CREB family of transcription factors (TFs), specifically interacting with the consensus ATF/CRE site ‘TGACGTCA’^[1].

Activating transcription factor 1 (ATF1) is a member of the ATF/CREB family of transcription factors (TFs), specifically interacting with the consensus ATF/CRE site ‘TGACGTCA’. CREB and ATF1 are required for t-Darpp upregulating Bcl-2 levels in resistance to ceramide-induced apoptosis in gastric cancer. Furthermore, expression of single chain antibody fragment anti-ATF1 in melanoma cells is found to suppresses the ATF1 tumorigenicity and metastatic potential in nude mice^[1].

Human

E. coli

C-6*His

P18846 (M1-V271)

466  [NCBI]

MEDSHKSTTSETAPQPGSAVQGAHISHIAQQVSSLSESEESQDSSDSIGSSQKAHGILARRPSYRKILKDLSSEDTRGRKGDGENSGVSAAVTSMSVPTPIYQTSSGQYIAIAPNGALQLASPGTDGVQGLQTLTMTNSGSTQQGTTILQYAQTSDGQQILVPSNQVVVQTASGDMQTYQIRTTPSATSLPQTVVMTSPVTLTSQTTKTDDPQLKREIRLMKNREAARECRRKKKEYVKCLENRVAVLENQNKTLIEELKTLKDLYSNKSVHHHHHH

Approximately 34.8 kDa

Greater than 95% as determined by reducing SDS-PAGE.

Lyophilized powder.

Lyophilized after extensive dialysis against PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.

• [1]. Guo-Liang Huang, et al. The protein level and transcription activity of activating transcription factor 1 is regulated by prolyl isomerase Pin1 in nasopharyngeal carcinoma progression. Cell Death Dis. 2016 Dec 29;7(12):e2571.  [Content Brief]