Azoreductase/NQO1 Protein, Human (His)

Azo reductase/NQO1 protein is a flavin-containing quinone reductase that uses NADH or NADPH to catalyze the two-electron reduction of quinone to hydroquinone. It regulates cellular redox status by detoxifying quinones and reducing plasma membrane redox components. Azoreductase/NQO1 Protein, Human (His) is the recombinant human-derived Azoreductase/NQO1 protein, expressed by E. coli , with N-His labeled tag. The total length of Azoreductase/NQO1 Protein, Human (His) is 274 a.a., with molecular weight of ~33 kDa.

Azoreductase/NQO1 protein is a flavin-containing quinone reductase that facilitates the two-electron reduction of quinones to hydroquinones, utilizing either NADH or NADPH as electron donors. Operating through a ping-pong kinetic mechanism, the electrons are sequentially transferred from NAD(P)H to the flavin cofactor and subsequently to the quinone, effectively bypassing the generation of semiquinone and reactive oxygen species. This enzymatic activity plays a crucial role in regulating cellular redox balance by detoxifying quinones. Azoreductase/NQO1 serves as a superoxide scavenger, preventing hydroquinone oxidation and supporting antioxidant defense mechanisms. Moreover, it participates in the activation of quinones, generating redox-reactive hydroquinones with potential antitumor properties through DNA cross-linking. Notably, the protein acts as a gatekeeper for the core 20S proteasome, interacting with tumor suppressors TP53 and TP73 in a NADH-dependent manner to inhibit their ubiquitin-independent degradation during oxidative stress.

Biological activity has been tested in vitro.

Human

E. coli

N-His

P15559-1 (M1-K274)

1728  [NCBI]

MVGRRALIVLAHSERTSFNYAMKEAAAAALKKKGWEVVESDLYAMNFNPIISRKDITGKLKDPANFQYPAESVLAYKEGHLSPDIVAEQKKLEAADLVIFQFPLQWFGVPAILKGWFERVFIGEFAYTYAAMYDKGPFRSKKAVLSITTGGSGSMYSLQGIHGDMNVILWPIQSGILHFCGFQVLEPQLTYSIGHTPADARIQILEGWKKRLENIWDETPLYFAPSSLFDLNFQAGFLMKKEVQDEEKNKKFGLSVGHHLGKSIPTDNQIKARK

Approximately 33 kDa

Greater than 90% as determined by reducing SDS-PAGE.

Lyophilized powder.

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.