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B2M/Beta-2 microglobulin Protein, Human (HEK293, His, solution)

Cat. No.: HY-P74404
COA Handling Instructions

B2M is a component of MHC class I complexes that present peptide antigens to the immune system. B2M/Beta-2 microglobulin Protein, Human (HEK293, His, solution) is the recombinant human-derived B2M/Beta-2 microglobulin protein, expressed by HEK293 , with C-His labeled tag. The total length of B2M/Beta-2 microglobulin Protein, Human (HEK293, His, solution) is 99 a.a., with molecular weight of ~13.5 kDa.

For research use only. We do not sell to patients.

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Description

B2M is a component of MHC class I complexes that present peptide antigens to the immune system. B2M/Beta-2 microglobulin Protein, Human (HEK293, His, solution) is the recombinant human-derived B2M/Beta-2 microglobulin protein, expressed by HEK293 , with C-His labeled tag. The total length of B2M/Beta-2 microglobulin Protein, Human (HEK293, His, solution) is 99 a.a., with molecular weight of ~13.5 kDa.

Background

B2M, or Beta-2-microglobulin, functions as a critical component of the class I major histocompatibility complex (MHC), playing a central role in presenting peptide antigens to the immune system. Notably, exogenously applied M. tuberculosis EsxA or EsxA-EsxB binds B2M and reduces its export to the cell surface, potentially leading to defects in class I antigen presentation. B2M exists as a heterodimer, composed of an alpha chain and a beta chain, with the latter serving as the beta-chain of major histocompatibility complex class I molecules. Polymers of B2M have been observed in tissues of patients on long-term hemodialysis. B2M, in its isolated form, interacts with M. tuberculosis EsxA and an EsxA-EsxB complex, forming a tripartite complex detectable in the host endoplasmic reticulum. The stability of the B2M-EsxA complex extends across a broad pH range and in the presence of high salt concentrations. Additionally, B2M forms heterotrimers with HLA-E, HLA-G, and HLA-F, along with a self- or foreign peptide, contributing to the diverse functions of the major histocompatibility complex. Furthermore, B2M engages in a heterotrimeric complex with MR1, playing a role in antigen presentation associated with metabolite antigens.

Species

Human

Source

HEK293

Tag

C-His

Accession

P61769 (I21-M119)

Gene ID

567  [NCBI]

Molecular Construction
N-term
B2M (I21-M119)
Accession # P61769
His
C-term
Synonyms
Beta-2-microglobulin; B2M
Molecular Weight

Approximately 13.5 kDa

Purity

Greater than 95% as determined by reducing SDS-PAGE.

Appearance

Solution.

Formulation

Supplied as a 0.2 μm filtered solution of PBS, pH 7.4.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

N/A.

Storage & Stability

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping

Shipping with dry ice.

Documentation

B2M/Beta-2 microglobulin Protein, Human (HEK293, His, solution) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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Product Name:
B2M/Beta-2 microglobulin Protein, Human (HEK293, His, solution)
Cat. No.:
HY-P74404
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