B7-H6 Protein, Human (Biotinylated, HEK293, His-Avi)

B7-H6, a distinctive monomeric protein, uniquely triggers NCR3-dependent natural killer (NK) cell activation, specifically interacting with NCR3 while avoiding engagement with other NK cell-activating receptors (NCR1, NCR2, and KLRK1). This specificity underscores B7-H6's unique role in initiating NK cell responses through the NCR3 pathway within the intricate network of NK cell activation mechanisms. B7-H6 Protein, Human (Biotinylated, HEK293, His-Avi) is the recombinant human-derived B7-H6 protein, expressed by HEK293 , with C-His, C-Avi labeled tag. The total length of B7-H6 Protein, Human (Biotinylated, HEK293, His-Avi) is 238 a.a., with molecular weight of 48-65 kDa.

B7-H6, a distinctive protein, serves as a trigger for NCR3-dependent natural killer (NK) cell activation. Operating as a monomer, B7-H6 exhibits a specific interaction with NCR3, distinctly avoiding engagement with other NK cell-activating receptors, such as NCR1, NCR2, and KLRK1. This interaction highlights its unique role in initiating NK cell responses through the NCR3 pathway, showcasing its specificity in the intricate network of NK cell activation mechanisms.

Human NKp30 hFc captured on CM5 Chip via Protein A can bind Biotinylated Human B7-H6 His-Avi with an affinity constant of 0.275 μM as determined in SPR assay.

Human

HEK293

C-His;C-Avi

Q68D85 (D25-S262)

374383  [NCBI]

48-65 kDa

Greater than 95% as determined by Tris-Bis PAGE.

Lyophilized powder.

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4. Normally 8% trehalose is added as protectant before lyophilization.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.