Butyrylcholinesterase/BCHE Protein, Human (HEK293, His)

Butyrylcholinesterase/BCHE Protein, Human (HEK293, His) is a recombinant human butyrylcholinesterase (BuChE) produced in HEK293 cells, with His tag. BuChE, one of cholinesterases, is a serine hydrolase closely related to acetylcholinesterase^[1].

Butyrylcholinesterase is a serine hydrolase closely related to acetylcholinesterase (AChE). Both enzymes have similar three-dimensional-structure. Though their catalytic mechanisms are similar, they display different substrate specificity and inhibitor sensitivity. BuChE is present in plasma and numerous vertebrate tissues. Unlike AChE that plays a key role in terminating the action of acetylcholine in cholinergic system, no direct involvement of BuChE in cholinergic system has been demonstrated. However, BuChE may be a surrogate for AChE in case of deficiency of this enzyme^[1].

Human

HEK293

C-6*His

P06276 (E29-L602)

590  [NCBI]

EDDIIIATKNGKVRGMNLTVFGGTVTAFLGIPYAQPPLGRLRFKKPQSLTKWSDIWNATKYANSCCQNIDQSFPGFHGSEMWNPNTDLSEDCLYLNVWIPAPKPKNATVLIWIYGGGFQTGTSSLHVYDGKFLARVERVIVVSMNYRVGALGFLALPGNPEAPGNMGLFDQQLALQWVQKNIAAFGGNPKSVTLFGESAGAASVSLHLLSPGSHSLFTRAILQSGSFNAPWAVTSLYEARNRTLNLAKLTGCSRENETEIIKCLRNKDPQEILLNEAFVVPYGTPLSVNFGPTVDGDFLTDMPDILLELGQFKKTQILVGVNKDEGTAFLVYGAPGFSKDNNSIITRKEFQEGLKIFFPGVSEFGKESILFHYTDWVDDQRPENYREALGDVVGDYNFICPALEFTKKFSEWGNNAFFYYFEHRSSKLPWPEWMGVMHGYEIEFVFGLPLERRDNYTKAEEILSRSIVKRWANFAKYGNPNETQNNSTSWPVFKSTEQKYLTLNTESTRIMTKLRAQQCRFWTSFFPKVLEMTGNIDEAEWEWKAGFHRWNNYMMDWKNQFNDYTSKKESCVGLHHHHHH

Approximately 90.0 kDa

Greater than 95% as determined by reducing SDS-PAGE.

Solution.

Supplied as a 0.2 μm filter solution of 20 mM TrisHCl, 150 mM NaCl, pH 7.5.

<1 EU/μg, determined by LAL method.

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.

• [1]. Patrick Masson, et al. Structure, activities and biomedical applications of human butyrylcholinesterase. Protein Pept Lett. 2009;16(10):1215-24.  [Content Brief]

  [2]. Patrick Masson, et al. Butyrylcholinesterase for protection from organophosphorus poisons; catalytic complexities and hysteretic behavior. Arch Biochem Biophys. 2010 Feb 15; 494(2): 107.  [Content Brief]