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Bcl-XL Protein, Mouse (His)

Cat. No.: HY-P72846
SDS COA Handling Instructions

Bcl-XL protein forms heterodimers with BAX, BAK, or BCL2, but its antiapoptotic activity does not require heterodimerization with BAX. Bcl-XL Protein, Mouse (His) is the recombinant mouse-derived Bcl-XL protein, expressed by E. coli , with C-His labeled tag. The total length of Bcl-XL Protein, Mouse (His) is 212 a.a., with molecular weight of ~33 kDa.

For research use only. We do not sell to patients.

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Size Price Stock Quantity
5 μg $45 In-stock
10 μg $78 In-stock
50 μg $220 In-stock
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Description

Bcl-XL protein forms heterodimers with BAX, BAK, or BCL2, but its antiapoptotic activity does not require heterodimerization with BAX. Bcl-XL Protein, Mouse (His) is the recombinant mouse-derived Bcl-XL protein, expressed by E. coli , with C-His labeled tag. The total length of Bcl-XL Protein, Mouse (His) is 212 a.a., with molecular weight of ~33 kDa.

Background

Bcl-XL protein forms heterodimers with BAX, BAK, or BCL2, with heterodimerization with BAX not being essential for its anti-apoptotic activity. Additionally, it interacts with isoform 1 of SIVA1, inhibiting its anti-apoptotic function. The protein also engages with IKZF3 and RTL10/BOP. Furthermore, interactions with DNM1L and CLTA suggest the potential formation of a complex in synaptic vesicles that includes clathrin and MFF. Notably, Bcl-XL interacts with NLRP1, specifically via the loop between motifs BH4 and BH3, but does not engage with NLRP2, NLRP3, NLRP4, PYCARD, or MEFV. It also interacts with BECN1, indicating its involvement in autophagy regulation.

Biological Activity

1. Immobilized human BID at 10 μg/mL (100 μl/well) can bind biotinylated mouse BCL2L1, The EC50 of biotinylated mouse BCL2L1 is 5.6 ng/mL.
2. Immobilized mouse BID at 10 μg/mL (100 μl/well) can bind biotinylated mouse BCL2L1, The EC50 of biotinylated mouse BCL2L1 is 7.1 ng/mL.

Species

Mouse

Source

E. coli

Tag

C-His

Accession

Q64373-1 (M1-R212)

Gene ID
Molecular Construction
N-term
Bcl-XL (M1-R212)
Accession # Q64373-1
His
C-term
Synonyms
Bcl-2-like protein 1; Bcl2-L-1; Apoptosis regulator Bcl-X; Bclx
AA Sequence

MSQSNRELVVDFLSYKLSQKGYSWSQFSDVEENRTEAPEETEAERETPSAINGNPSWHLADSPAVNGATGHSSSLDAREVIPMAAVKQALREAGDEFELRYRRAFSDLTSQLHITPGTAYQSFEQVVNELFRDGVNWGRIVAFFSFGGALCVESVDKEMQVLVSRIASWMATYLNDHLEPWIQENGGWDTFVDLYGNNAAAESRKGQERFNR

Molecular Weight

Approximately 33 kDa

Purity

Greater than 95% as determined by reducing SDS-PAGE

Appearance

Solution

Formulation

Supplied as a 0.2 μm filtered solution of PBS, pH 7.5.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

N/A.

Storage & Stability

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping

Shipping with dry ice.

Documentation

Bcl-XL Protein, Mouse (His) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

  • Specific Activity Calculator

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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Bcl-XL Protein, Mouse (His)
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HY-P72846
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