BPI Protein, Human (HEK293, His)

BPI Protein, Human (HEK293, His), a member of a genomically conserved lipid-interactive protein family, belongs to a diverse group of polypeptides with antimicrobial activity that are thought to participate in antimicrobial host defence^[1][2][3].

BPI consists of two functionally distinct domains: a potently antibacterial and anti-endotoxin amino-terminal domain (-20 kDa) and a carboxy-terminal portion that imparts opsonic activity to BPI^[1].
Bactericidal/permeability-increasing protein has endotoxin-neutralizing activity^[2].
BPI exhibits strong anti-microbial activity against GNB and potent endotoxin-neutralizing activity^[3].

Measured by its ability to inhibit LPS-induced IL-8 secretion by THP-1 human acute monocytic leukemia cell. The ED₅₀ for this effect is 3.082 ng/mL in the present of 2 μg/mL LPS, corresponding to a specific activity is 3.245×10⁵ U/mg.

Human

HEK293

C-6*His

AAH40955.1 (V32-K487)

671  [NCBI]

VNPGVVVRISQKGLDYASQQGTAALQKELKRIKIPDYSDSFKIKHLGKGHYSFYSMDIREFQLPSSQISMVPNVGLKFSISNANIKISGKWKAQKRFLKMSGNFDLSIEGMSISADLKLGSNPTSGKPTITCSSCSSHINSVHVHISKSKVGWLIQLFHKKIESALRNKMNSQVCEKVTNSVSSKLQPYFQTLPVMTKIDSVAGINYGLVAPPATTAETLDVQMKGEFYSENHHNPPPFAPPVMEFPAAHDRMVYLGLSDYFFNTAGLVYQEAGVLKMTLRDDMIPKESKFRLTTKFFGTFLPEVAKKFPNMKIQIHVSASTPPHLSVQPTGLTFYPAVDVQAFAVLPNSSLASLFLIGMHTTGSMEVSAESNRLVGELKLDRLLLELKHSNIGPFPVELLQDIMNYIVPILVLPRVNEKLQKGFPLPTPARVQLYNVVLQPHQNFLLFGADVVYKHHHHHH

50-60 kDa

Greater than 95% as determined by reducing SDS-PAGE.

Lyophilized powder.

Lyophilized after extensive dialysis against 20 mM PB,150 mM NaCl, pH7.4 or 4mM HCl.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.

• [1]. P Elsbach, et al. Role of the bactericidal/permeability-increasing protein in host defence. Curr Opin Immunol. 1998 Feb;10(1):45-9.  [Content Brief]

  [2]. M N Marra, et al. Bactericidal/permeability-increasing protein has endotoxin-neutralizing activity. J Immunol. 1990 Jan 15;144(2):662-6.  [Content Brief]

  [3]. Hendrik Schultz, et al. The bactericidal/permeability-increasing protein (BPI) in infection and inflammatory disease. Clin Chim Acta. 2007 Sep;384(1-2):12-23.  [Content Brief]