BTK Protein, Human (Biotinylated, sf9, His, Avi)

The BTK protein is a key non-receptor tyrosine kinase that is essential for B lymphocyte development and signaling. After BCR activation, BTK initiates a cascade reaction, phosphorylates PLCG2 and activates downstream pathways, affecting calcium mobilization and PKC activation. BTK Protein, Human (Biotinylated, sf9, His, Avi) is the recombinant human-derived BTK, expressed by Sf9 insect cells , with His, Avi labeled tag. ,

BTK Protein, a non-receptor tyrosine kinase, is indispensable for B lymphocyte development, differentiation, and signaling. Upon antigen binding to the B-cell antigen receptor (BCR), BTK triggers a signaling cascade leading to B-cell activation, phosphorylating PLCG2 and initiating downstream pathways involving calcium mobilization and protein kinase C (PKC) activation. Serving as a platform for diverse signaling proteins, BTK is implicated in cytokine receptor signaling pathways and is crucial in innate and adaptive immunity, particularly within the Toll-like receptors (TLR) pathway. It plays a critical role in regulating TLR9 activation in splenic B-cells and induces the activity of NF-kappa-B, contributing to the transcriptional regulation of numerous genes. Additionally, BTK activates NLRP3 inflammasome assembly, phosphorylates transcription factor GTF2I, and is involved in apoptosis regulation, highlighting its multifaceted functions in immune response and cellular processes.

The activity was measured by off-chip mobility shift assay(MSA). The enzyme was incubated with fluorecence-labeled substrate and Mg(or Mn)/ATP. The phosphorylated and unphosphorylated substrates were separated and detected by MSA device. The Km of BTK for its substrate EGFRtide is 137 μM.

Human

Sf9 insect cells

His;Avi

Q06187-1 (A2-S659, C481S)

695

80.4 kDa

Greater than 90% as determined by reducing SDS-PAGE.

Solution

Supplied as a 0.2 μm filtered solution of 50 mM Tris-HCl, 150 mM NaCl, 10% glycerol, 1 mM DTT, pH 7.5 or 30 mM HEPES (pH 7.5), 150 mM NaCl, 1 mM DTT, 10% glycerol.

<1 EU/μg, determined by LAL method.

Please use rapid thawing with running water to thaw the protein.

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.