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  4. CALCB Protein, Human (HEK293, hFc)

CALCB Protein, Human (HEK293, hFc)

Cat. No.: HY-P7706
COA Handling Instructions

CALCB Protein, Human (HEK293, hFc), a recombinant human-derived CALCB protein, produced in HEK293 cells, has a hFc fragment at the C-terminus.

For research use only. We do not sell to patients.

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Size Price Stock Quantity
10 μg $60 In-stock
50 μg $180 In-stock
100 μg   Get quote  

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Top Publications Citing Use of Products
  • Biological Activity

  • Technical Parameters

  • Properties

  • Documentation

  • References

Description

CALCB Protein, Human (HEK293, hFc), a recombinant human-derived CALCB protein, produced in HEK293 cells, has a hFc fragment at the C-terminus.

Background

Calcitonin gene-related peptide (CGRP) has two major forms (α and β). It belongs to a group of peptides that all act on an unusual receptor family. These receptors consist of calcitonin receptor-like receptor (CLR) linked to an essential receptor activity modifying protein (RAMP) that is necessary for full functionality. CGRP is a highly potent vasodilator and, partly as a consequence, possesses protective mechanisms that are important for physiological and pathological conditions involving the cardiovascular system and wound healing[1].

Species

Human

Source

HEK293

Tag

C-hFc

Accession

P10092 (A26-F118)

Gene ID

797  [NCBI]

Molecular Construction
N-term
CALCB (A26-F118)
Accession # P10092
hFc
C-term
Synonyms
rHuCALC2, C-Fc; Calcitonin gene-related peptide 2; Calcitonin gene-related peptide II; CALCB
AA Sequence

APFRSALESSPDPATLSKEDARLLLAALVQDYVQMKASELKQEQETQGSSSAAQKRACNTATCVTHRLAGLLSRSGGMVKSNFVPTNVGSKAF-Fctag

Molecular Weight

38-42 kDa

Purity

Greater than 90% as determined by reducing SDS-PAGE.

Appearance

Lyophilized powder.

Formulation

Lyophilized after extensive dialysis against PBS, pH 7.4.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Storage & Stability

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Documentation
References

CALCB Protein, Human (HEK293, hFc) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

  • Specific Activity Calculator

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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CALCB Protein, Human (HEK293, hFc)
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HY-P7706
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