Carboxypeptidase A2/CPA2 Protein, Human (HEK293, His)

Carboxypeptidase A2/CPA2 Protein, Human (HEK293, His) is an approximately 50.0 kDa human carboxypeptidase A2 with a His-flag. Carboxypeptidase A2 is a secreted pancreatic procarboxy -peptidase, and cleaves the C-terminal amide or ester bond of peptides.

There exists different forms of human pancreatic procarboxypeptidase A, and the A1 and A2 forms are always secreted as monomeric proteins with different biochemical properties^[1].
The hydrolytic action of CPA2 prefers towards long substrates with aromatic amino acids in their C-terminal end, particularly tryptophan. CPA2 comprises a signal peptide, a pro region and a mature chain, and can be activated after cleavage of the pro peptide^[2].
Carboxypeptidase A2 is a secreted pancreatic procarboxy-peptidase, and cleaves the C-terminal amide or ester bond of peptides that have a free C-terminal carboxyl group^[1][2].

Human

HEK293

C-6*His

AAP36067.1 (L17-Y417)

1358  [NCBI]

LETFVGDQVLEIVPSNEEQIKNLLQLEAQEHLQLDFWKSPTTPGETAHVRVPFVNVQAVKVFLGSQGIAYSIMIEDVQVLLDKENEEMLFNRRRERSGNFNFGAYHTLEEISQEMDNLVAEHPGLVSKVNIGSSFENRPMNVLKFSTGGDKPAIWLDAGIHAREWVTQATALWTANKIVSDYGKDPSITSILDALDIFLLPVTNPDGYVFSQTKNRMWRKTRSKVSGSLCVGVDPNRNWDAGFGGPGASSNPCSDSYHGPSANSEVEVKSIVDFIKSHGKVKAFITLHSYSQLLMFPYGYKCTKLDDFDELSEVAQKAAQSLRSLHGTKYKVGPICSVIYQASGGSIDWSYDYGIKYSFAFELRDTGRYGFLLPARQILPTAEETWLGLKAIMEHVRDHPYHHHHHH

Approximately 50.0 kDa

Greater than 95% as determined by reducing SDS-PAGE.

<1 EU/μg, determined by LAL method.

• [1]. L Catasús, et al. The sequence and conformation of human pancreatic procarboxypeptidase A2. cDNA cloning, sequence analysis, and three-dimensional model. J Biol Chem  [Content Brief]

  [2]. P Aloy, et al. Comparative analysis of the sequences and three-dimensional models of human procarboxypeptidases A1, A2 and B. Biol Chem. 1998 Feb;379(2):149-55.  [Content Brief]