CD162/PSGL-1 Protein, Mouse (HEK293, Fc)

CD162/PSGL-1 Protein, Mouse (HEK293, Fc) is a recombinant mouse CD162 expressed in HEK 293 cells with a C-Fc tag at the C-terminus. CD162/PSGL-1 Protein plays an important role in immunity and homeostasis ^[1][2].

PSGL-1 (P-Selectin Glycoprotein Ligand-1, designated CD162), is a type I membrane protein. PSGL-1 is expressed as a disulfide-linked homodimer with an extended mucin ectodomain composed of serine, threonine, and proline-rich decameric repeats bearing sites for potential O-glycan modification. PSGL-1 is expressed on all myeloid and lymphoid lineages including dendritic cells (DCs) and is downregulated on lymphocytes after entry into secondary lymphoid organs. Although not originally observed in megakaryocytes, PSGL-1 expression was identified in platelets with expression further increased in inflammatory settings^[1][2].

Mouse

HEK293

C-Fc

CAA62583.1 (Q42-C307)

20345  [NCBI]

QVVGDDDFEDPDYTYNTDPPELLKNVTNTVAAHPELPTTVVMLERDSTSAGTSERATEKIATTDPTAPGTGGTAVGMLSTDSATQWSLTSVETVQPASTEVETSQPAPMEAETSQPAPMEAETSQPAPMEADTSKPAPTEAETSKPAPTEAETSQPAPNEAETSKPAPTEAETSKPAPTEAETTQLPRIQAVKTLFTTSAATEVPSTEPTTMETASTESNESTIFLGPSVTHLPDSGLKKGLIVTPGNSPAPTLPGSSDLIPVKQC-Fctag

Approximately 90 kDa

Greater than 90% as determined by reducing SDS-PAGE.

Lyophilized powder.

Lyophilized after extensive dialysis against 20 mM PB,150 mM NaCl, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.

• [1]. Yang J, et, al. The biology of P-selectin glycoprotein ligand-1: its role as a selectin counterreceptor in leukocyte-endothelial and leukocyte-platelet interaction. Thromb Haemost. 1999 Jan;81(1):1-7.  [Content Brief]

  [2]. Carlow DA, et, al. PSGL-1 function in immunity and steady state homeostasis. Immunol Rev. 2009 Jul;230(1):75-96.  [Content Brief]