CD26/Dipeptidyl Peptidase 4 Protein, Mouse (HEK293, Fc)

CD26/dipeptidyl peptidase 4 is a key cell surface receptor for T cell activation. It binds to ADA, CAV1, IGF2R and PTPRC to promote T cell proliferation and NF-kappa-B activation. CD26/Dipeptidyl Peptidase 4 Protein, Mouse (HEK293, Fc) is the recombinant mouse-derived CD26/Dipeptidyl Peptidase 4 protein, expressed by HEK293 , with N-hFc labeled tag. The total length of CD26/Dipeptidyl Peptidase 4 Protein, Mouse (HEK293, Fc) is 732 a.a., with molecular weight of ~122.5 kDa.

The CD26/Dipeptidyl Peptidase 4 protein serves as a cell surface glycoprotein receptor crucial for the costimulatory signal essential in T-cell receptor (TCR)-mediated T-cell activation. Functioning as a positive regulator of T-cell coactivation, CD26 binds to ADA, CAV1, IGF2R, and PTPRC. Its interactions with CAV1 and CARD11 induce T-cell proliferation and NF-kappa-B activation in a T-cell receptor/CD3-dependent manner. Additionally, CD26's association with ADA regulates lymphocyte-epithelial cell adhesion, while its partnership with FAP contributes to pericellular proteolysis of the extracellular matrix (ECM), facilitating the migration and invasion of endothelial cells into the ECM. CD26 may also play a role in promoting lymphatic endothelial cell adhesion, migration, and tube formation. When overexpressed, CD26 enhances cell proliferation, a process inhibited by GPC3. Beyond its coactivation role, CD26 functions as a serine exopeptidase with dipeptidyl peptidase activity, regulating various physiological processes by cleaving peptides in the circulation, including chemokines, mitogenic growth factors, neuropeptides, and peptide hormones, specifically targeting peptides with unsubstituted N-termini when the penultimate residue is proline.

Measured by its ability to cleave the fluorogenic peptide substrate, Gly-Pro-7-amido-4-methylcoumarin (GP-AMC). Read at excitation and emission wavelengths of 380 nm and 460 nm .The specific activity is 1362.0674 pmol/min/µg, as measured under the described conditions.

Mouse

HEK293

N-hFc

P28843 (S29-H760)

13482  [NCBI]

SKDEAAADSRRTYSLADYLKSTFRVKSYSLWWVSDFEYLYKQENNILLLNAEHGNSSIFLENSTFESFGYHSVSPDRLFVLLEYNYVKQWRHSYTASYNIYDVNKRQLITEEKIPNNTQWITWSPEGHKLAYVWKNDIYVKVEPHLPSHRITSTGEENVIYNGITDWVYEEEVFGAYSALWWSPNNTFLAYAQFNDTGVPLIEYSFYSDESLQYPKTVWIPYPKAGAVNPTVKFFIVNIDSLSSSSSAAPIQIPAPASVARGDHYLCDVVWATEERISLQWLRRIQNYSVMAICDYDKINLTWNCPSEQQHVEMSTTGWVGRFRPAEPHFTSDGSSFYKIISDKDGYKHICHFPKDKKDCTFITKGAWEVISIEALTSDYLYYISNQYKEMPGGRNLYKIQLTDHTNVKCLSCDLNPERCQYYAVSFSKEAKYYQLGCWGPGLPLYTLHRSTDHKELRVLEDNSALDRMLQDVQMPSKKLDFIVLNETRFWYQMILPPHFDKSKKYPLLLDVYAGPCSQKADASFRLNWATYLASTENIIVASFDGRGSGYQGDKIMHAINRRLGTLEVEDQIEAARQFVKMGFVDSKRVAIWGWSYGGYVTSMVLGSGSGVFKCGIAVAPVSRWEYYDSVYTERYMGLPIPEDNLDHYRNSTVMSRAEHFKQVEYLLIHGTADDNVHFQQSAQISKALVDAGVDFQAMWYTDEDHGIASSTAHQHIYSHMSHFLQQCFSLH

Approximately 122.5 kDa

• 
  Greater than 95% as determined by reducing SDS-PAGE.

Lyophilized powder.

Lyophilized from a 0.2 μm filtered solution of 20 mM PB, 150 mM NaCl, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.