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  5. CD28 Protein, Mouse (Biotinylated , HEK293, His)

CD28 Protein, Mouse (Biotinylated , HEK293, His)

Cat. No.: HY-P74311
Handling Instructions

CD28 Protein, a crucial regulator of T-cell activation, promotes cell proliferation, cytokine production, and T-cell survival. It enhances IL4 and IL10 production when combined with TCR/CD3 ligation and CD40L costimulation. CD28 Protein exists as a disulfide-linked homodimer and interacts with DUSP14, CD80/B7-1, CD86/B7-2/B70, and GRB2 (By similarity). CD28 Protein, Mouse (Biotinylated, HEK293, His) is the recombinant mouse-derived CD28 protein, expressed by HEK293 , with C-His labeled tag. The total length of CD28 Protein, Mouse (Biotinylated, HEK293, His) is 130 a.a., with molecular weight of 32-35 kDa.

For research use only. We do not sell to patients.

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  • Biological Activity

  • Technical Parameters

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  • Documentation

Description

CD28 Protein, a crucial regulator of T-cell activation, promotes cell proliferation, cytokine production, and T-cell survival. It enhances IL4 and IL10 production when combined with TCR/CD3 ligation and CD40L costimulation. CD28 Protein exists as a disulfide-linked homodimer and interacts with DUSP14, CD80/B7-1, CD86/B7-2/B70, and GRB2 (By similarity). CD28 Protein, Mouse (Biotinylated, HEK293, His) is the recombinant mouse-derived CD28 protein, expressed by HEK293 , with C-His labeled tag. The total length of CD28 Protein, Mouse (Biotinylated, HEK293, His) is 130 a.a., with molecular weight of 32-35 kDa.

Background

CD28 Protein, a key player in T-cell activation, is involved in inducing cell proliferation, cytokine production, and promoting T-cell survival. It enhances the production of IL4 and IL10 in T-cells when combined with TCR/CD3 ligation and CD40L costimulation. CD28 Protein exists as a homodimer through disulfide-linkage and interacts with DUSP14. It binds to CD80/B7-1 and CD86/B7-2/B70, and also has an interaction with GRB2 (By similarity).

Species

Mouse

Source

HEK293

Tag

C-His

Accession

P31041 (N20-K149)

Gene ID
Molecular Construction
N-term
CD28 (N20-K149)
Accession # P31041
His
C-term
Synonyms
CD28; T-cell-specific surface glycoprotein CD28; Tp44
Molecular Weight

32-35 kDa

Purity

Greater than 95% as determined by reducing SDS-PAGE.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Documentation
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

  • Specific Activity Calculator

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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CD28 Protein, Mouse (Biotinylated , HEK293, His)
Cat. No.:
HY-P74311
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